Micro axial tomography: A miniaturized, versatile stage device
to overcome resolution anisotropy in fluorescence light
microscopy

J 2011

Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK et. al.

Základní údaje

Originální název

Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy

Autoři

STAIER, Florian (276 Německo), Heinz EIPEL (276 Německo), Petr MATULA (203 Česká republika, domácí), Alexei V EVSIKOV (643 Rusko), Michal KOZUBEK (203 Česká republika, garant, domácí), Christoph CREMER (276 Německo) a Michael HAUSMANN (276 Německo)

Vydání

Rev. Sci. Instrum. USA, American Institute of Physica, 2011, 0034-6748

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

20200 2.2 Electrical engineering, Electronic engineering, Information engineering

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.367

Kód RIV

RIV/00216224:14330/11:00053149

Organizační jednotka

Fakulta informatiky

UT WoS

000295621100023

Klíčová slova anglicky

fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors

Štítky

cbia-web

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 12. 2. 2018 15:10, doc. RNDr. Petr Matula, Ph.D.

Anotace

V originále

To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.

Návaznosti

MSM0021622419, záměr

Název: Vysoce paralelní a distribuované výpočetní systémy

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vysoce paralelní a distribuované výpočetní systémy

2B06052, projekt VaV

Název: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Akronym: Biomarker)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů

Citovat

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER a Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. Rev. Sci. Instrum. USA: American Institute of Physica, 2011, roč. 82, č. 9, s. "093701-1"-"093701-8", 8 s. ISSN 0034-6748.

@article{950311,
   author = {Staier, Florian and Eipel, Heinz and Matula, Petr and Evsikov, Alexei V and Kozubek, Michal and Cremer, Christoph and Hausmann, Michael},
   article_location = {USA},
   article_number = {9},
   keywords = {fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors},
   language = {eng},
   issn = {0034-6748},
   journal = {Rev. Sci. Instrum.},
   title = {Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy},
   volume = {82},
   year = {2011}
}

TY  - JOUR
ID  - 950311
AU  - Staier, Florian - Eipel, Heinz - Matula, Petr - Evsikov, Alexei V - Kozubek, Michal - Cremer, Christoph - Hausmann, Michael
PY  - 2011
TI  - Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy
JF  - Rev. Sci. Instrum.
VL  - 82
IS  - 9
SP  - "093701-1"-"093701-8"
EP  - "093701-1"-"093701-8"
PB  - American Institute of Physica
SN  - 00346748
KW  - fluorescence
KW  - glass fibres
KW  - image reconstruction
KW  - image resolution
KW  - optical microscopes
KW  - optical microscopy
KW  - optical tomography
KW  - stepping motors
N2  - To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.
ER  -

STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER a Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. \textit{Rev. Sci. Instrum.}. USA: American Institute of Physica, 2011, roč.~82, č.~9, s.~''093701-1''-''093701-8'', 8 s. ISSN~0034-6748.

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