| STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER a Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. Rev. Sci. Instrum. USA: American Institute of Physica, 2011, roč. 82, č. 9, s. "093701-1"-"093701-8", 8 s. ISSN 0034-6748. |
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@article{950311,
author = {Staier, Florian and Eipel, Heinz and Matula, Petr and Evsikov, Alexei V and Kozubek, Michal and Cremer, Christoph and Hausmann, Michael},
article_location = {USA},
article_number = {9},
keywords = {fluorescence; glass fibres; image reconstruction; image resolution; optical microscopes; optical microscopy; optical tomography; stepping motors},
language = {eng},
issn = {0034-6748},
journal = {Rev. Sci. Instrum.},
title = {Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy},
volume = {82},
year = {2011}
}
TY - JOUR
ID - 950311
AU - Staier, Florian - Eipel, Heinz - Matula, Petr - Evsikov, Alexei V - Kozubek, Michal - Cremer, Christoph - Hausmann, Michael
PY - 2011
TI - Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy
JF - Rev. Sci. Instrum.
VL - 82
IS - 9
SP - "093701-1"-"093701-8"
EP - "093701-1"-"093701-8"
PB - American Institute of Physica
SN - 00346748
KW - fluorescence
KW - glass fibres
KW - image reconstruction
KW - image resolution
KW - optical microscopes
KW - optical microscopy
KW - optical tomography
KW - stepping motors
N2 - To overcome anisotropic optical resolution or point localization precision in 3D fluorescence microscopy, micro axial tomography was suggested which allows object tilting on the microscopic stage. Here, we present a miniaturized device which can be implemented in a motor driven microscope stage. The footprint of this device corresponds to a standard microscope slide. A special glass fiber can manually be adjusted in the object space of the microscope lens. A stepwise fiber rotation can be controlled by a miniaturized stepping motor incorporated into the device. Test particles were fixed onto glass fibers, optically localized with high precision, and automatically rotated to obtain views from different perspective angles under which distances of corresponding pairs of objects were determined. From these angle dependent distance values, the real 3D distance was calculated with a precision in the ten nanometer range using standard microscopic equipment. Only very few images registered under different rotation angles are sufficient for full 3D reconstruction.
ER -
STAIER, Florian, Heinz EIPEL, Petr MATULA, Alexei V EVSIKOV, Michal KOZUBEK, Christoph CREMER a Michael HAUSMANN. Micro axial tomography: A miniaturized, versatile stage device to overcome resolution anisotropy in fluorescence light microscopy. \textit{Rev. Sci. Instrum.}. USA: American Institute of Physica, 2011, roč.~82, č.~9, s.~''093701-1''-''093701-8'', 8 s. ISSN~0034-6748.
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