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@misc{951869,
author = {Dubový, Petr},
booktitle = {Joint Conference of the Czech and Slovak Neuroscience Societies, Smolenice},
language = {eng},
title = {Is cytokine synthesis in the dorsal root ganglia related to mechanoallodynia and thermal hyperalgesia in the rat model of neuropathic pain?},
year = {2011}
}
TY - SLIDE
ID - 951869
AU - Dubový, Petr
PY - 2011
TI - Is cytokine synthesis in the dorsal root ganglia related to mechanoallodynia and thermal hyperalgesia in the rat model of neuropathic pain?
N2 - Many experimental studies have documented a correlation of pro-inflammatory cytokine elevations with induction and maintenance of neuropathic pain. In our experiments, Wistar rats operated on unilateral chronic constriction injury (CCI) of the sciatic nerve and sham-operated rats were allowed to survive for 1, 3, 7 and 14 days. Withdrawal thresholds for mechanical allodynia and thermal hyperalgesia were measured in the ipsi- and contralateral hind and fore paws. Expression of TNFa and IL-6 mRNAs was investigated by in situ hybridization and quantified by means of qRT-PCR in L4-L5 (homonymous) and C7-C8 (heteronymous) dorsal root ganglia (DRG). Bilateral up-regulation of TNFa and IL-6 mRNAs was found in the neurons and satellite glial cells of DRG from all investigated segments. TNFa mRNA was peaked in L4-L5 DRG at one day while IL-6 mRNA three days from CCI. Elevation of TNFa and IL-6 mRNAs in C7-C8 DRG was of lower magnitude when compared with L4-L5 DRG. An increased level of cytokine mRNAs was measured and in situ hybridization staining was found in satellite glial cells of DRG from sham-operated animals. The changes of TNFa and IL-6 mRNAs in DRG did not correlate with mechanical allodynia and thermal hyperalgesia.
ER -
DUBOVÝ, Petr. Is cytokine synthesis in the dorsal root ganglia related to mechanoallodynia and thermal hyperalgesia in the rat model of neuropathic pain? In \textit{Joint Conference of the Czech and Slovak Neuroscience Societies, Smolenice}. 2011.
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