GELBÍČOVÁ, Tereza and Renata KARPÍŠKOVÁ. Outdoor environment as a source of Listeria monocytogenes in food chain. Czech Journal of Food Sciences. 2012, vol. 30, No 1, p. 83-88. ISSN 1212-1800.
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Basic information
Original name Outdoor environment as a source of Listeria monocytogenes in food chain
Name in Czech Vnější prostředí jako zdroj Listeria monocytogenes v potravinovém řetězci
Authors GELBÍČOVÁ, Tereza (203 Czech Republic, guarantor, belonging to the institution) and Renata KARPÍŠKOVÁ (203 Czech Republic).
Edition Czech Journal of Food Sciences, 2012, 1212-1800.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 21101 Food and beverages
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.685
RIV identification code RIV/00216224:14310/12:00081777
Organization unit Faculty of Science
UT WoS 000300598500011
Keywords (in Czech) virulence; listerie; antimikrobiální rezistence; polymorfismus délky restrikčních fragmentů; pulzní gelová elektroforéza (PFGE)
Keywords in English virulence; listeria; antimicrobial resistance; restriction fragment length polymorphism; pulsed-field gel electrophoresis (PFGE)
Tags AKR, rivok
Changed by Changed by: Ing. Nicole Zrilić, učo 240776. Changed: 24/5/2017 16:57.
Abstract
The aims of this study were to monitor the presence of Listeria monocytogenes in environmental sources and to evaluate the phenotypic and molecular characteristics of the isolates recovered. L. monocytogenes was isolated in 12 (11.2%) of the 107 samples from the wild, farm environment, and vegetation. Most isolates (83.3%) were of serotype 1/2a and the remainder (2) were of serotype 4b. All 12 isolates were susceptible to the whole range of antimicrobials tested. These12 strains were carriers of the virulence genes prfA, hlyA, actA, plcA, plcB, inlA, inlB, inlC, and inlJ. The detection of the inlA gene in 4 (33.3%) of 12 strains using the PCR-RFLP suggests the potential of some of these strains to penetrate into epithelial cells of the intestinal barrier. Macrorestriction analysis also confirmed clonal identity of some environmental isolates with food and human isolates. These results indicate that the external environment is a source of potentially pathogenic strains of L. monocytogenes.
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