In vivo determination of hydroxyl radicals in isolated guinea pig hearts during ischemia and reperfusion

PAULOVÁ, Hana, Tibor STRAČINA, Marie NOVÁKOVÁ and Eva TÁBORSKÁ. In vivo determination of hydroxyl radicals in isolated guinea pig hearts during ischemia and reperfusion. In 11th International Nutrition and Diagnostics Conference INDC 2011. 2011. ISBN 978-80-7395-378-2.

Basic information

• Original name: In vivo determination of hydroxyl radicals in isolated guinea pig hearts during ischemia and reperfusion
• Name in Czech: In vivo stanovení hydroxylových radikálů v modelu isolovaného srdce morčete během ischemie a reperfuze
• Authors: PAULOVÁ, Hana (203 Czech Republic, guarantor, belonging to the institution), Tibor STRAČINA (703 Slovakia, belonging to the institution), Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution) and Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution).
• Edition: 11th International Nutrition and Diagnostics Conference INDC 2011, 2011.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14110/11:00053566
• Organization unit: Faculty of Medicine
• ISBN: 978-80-7395-378-2
• Keywords in English: isolated heart; guinea pig; hydroxyl radical; CK; LDH

Abstract

Myocardial ischemia-reperfusion injury is linked with production of oxygen free radicals. We evaluated the production of hydroxyl radical in isolated guinea pig hearts during ischemia and reperfusion. To investigate the other parameters of myocardial injury, we measured the creatine kinase, lactate dehydrogenase and lactate in collected effluents during both ischemia and reperfusion periods. The increased levels of CK, LDH and lactate and the comparison of these parameters with production of hydroxyl radicals shows that the hydroxyl radicals generation play important role in ischemia-reperfusion injury.

Links

• MSM0021622402, plan (intention): Name: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministry of Education, Youth and Sports of the CR, Early diagnostics and treatment of cardiovascular diseases