2011
A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides
LUBAL, Přemysl, Sylvie HOLUBOVÁ a Libuše TRNKOVÁZákladní údaje
Originální název
A solution study of protonation chemical equlibria of nucleobases and short oligonucleotides
Autoři
LUBAL, Přemysl (203 Česká republika, garant, domácí), Sylvie HOLUBOVÁ (203 Česká republika, domácí) a Libuše TRNKOVÁ (203 Česká republika, domácí)
Vydání
Biopolymers: Structure and dynamics, A biophysical workshop, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10406 Analytical chemistry
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00053670
Organizační jednotka
Přírodovědecká fakulta
ISBN
978-80-263-0050-2
Klíčová slova anglicky
oligonucleotides;cytosine;adenine;molecular spectroscopy;equispec;opium
Změněno: 13. 4. 2012 12:24, prof. RNDr. Libuše Trnková, CSc.
Anotace
V originále
The protonation constants of nucleobases and relevant nucleosides and nucleotides were determined by means of potenciometry and molecular absorption spectroscopy in UV region (I=0.15 mol.l-1 NaCl,t=25C) and the experimental data were evaluated by OPIUM program. In addition, thermodynamic parameters for nucleobase ligands were estimated. Also, some examples of ligand complexation by transition metal ions (e.g. copper(II),zinc(II),kadmium(II)) will be presented and their importance for bioligand speciation will be discussed. The protonation behavior of ODN containing C, i.e.(CCC)n,n=1–3, was followed by means of molecular spectroscopy in pH region 2–8 and variable temperature and ionic strength adjusted by NaCl. The experimental data were treated by means of OPIUM, MCR – ALS and EQUISPEC software and distribution diagram of species as pH fuction were calculated. For comparison, the protonation behavior of ODN with the (CAC)3, A3C3C3 and C3A3C3 sequence was also investigated. The influence of the structural motif and length on protonation constants o studied ODN seems to be very important due to probace stabilization of the secondary structure of the biomacromolecule.
Návaznosti
LC06035, projekt VaV |
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ME09065, projekt VaV |
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MUNI/A/0992/2009, interní kód MU |
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