Association of Interleukin-8 Gene Polymorphisms with
Periodontitis and Subgingival Bacterial Colonization in the
Czech Population

a 2011

Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population

LINHARTOVÁ, Petra, Václav ŘEHKA, Vendula BARTÁKOVÁ, Antonín FASSMANN, Lydie IZAKOVIČOVÁ HOLLÁ et. al.

Základní údaje

Originální název

Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population

Autoři

LINHARTOVÁ, Petra, Václav ŘEHKA, Vendula BARTÁKOVÁ, Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ

Vydání

XV. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita, 2011

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

ISBN

978-80-210-5594-0

Změněno: 2. 11. 2011 17:25, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Anotace

V originále

Periodontitis is an inflammatory disease induced by colonization of the dentogingival interface with pathogenic bacteria and characterized by the connective tissue loss and alveolar bone destruction. Interleukin-8 (IL-8), a CXC chemokine with multiple biological functions, is an important inflammatory mediator responsible for the migration and activation of neutrophils. IL-8 is involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. The aim of this study was to associate the two single nucleotide polymorphisms in IL-8 with periodontitis and subgingival bacterial colonization in the Czech population. In this case-control association study, a total of 489 unrelated Czech subjects were included. Genomic DNA of 270 patients with chronic periodontitis (CP), 64 patients with aggressive periodontitis (AgP) and 155 healthy/non-periodontitis controls was genotyped using Real-time PCR method for IL-8 gene polymorphisms (RS4073 -251 T/A and RS2227307 +396 T/G). Subgingival bacterial colonization (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Peptostreptococcus micros, Fusobacterium nucleatum in subgingival pockets) was investigated by the DNA-microarray based on a periodontal pathogen detection kit in a subgroup of subjects (N=169). Allele and genotype frequencies of both investigated polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (P>0.05). However, F. nucleatum occurred less frequently in non-periodontitis individuals positive for the T allele of IL-8 +396 T/G variant (OR = 0.10, confidence interval (CI): 0.01-0.82, P<0.01). In contrast, IL-8 -251 T and +396 T allele carriers had an increased odds ratio (OR) for the individual presence of A. actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) in AgP patients (OR = 17.0, confidence interval (CI): 1.85-156.27, P<0.01 for -251 T/A and OR = 10.11, confidence interval (CI): 1.05-97.01, P<0.05 for +396 T/G). Although the IL-8 variants could be shown to be associated with subgingival colonization with A. actinomycetemcomitans in AgP, our findings indicate that these polymorphisms alone are not associated with susceptibility to CP or AgP in the Czech population. This study was supported by projects 1M0528 and IGA NT11405-6.

Návaznosti

NT11405, projekt VaV

Název: Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

Investor: Ministerstvo zdravotnictví ČR, Mikrobiologické a genetické determinanty rozvoje a progrese parodontitidy u diabetiků 1. a 2. typu a jejich reciproční vztah ke kompenzaci diabetu

1M0528, projekt VaV

Název: Stomatologické výzkumné centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Stomatologické výzkumné centrum

Citovat

LINHARTOVÁ, Petra, Václav ŘEHKA, Vendula BARTÁKOVÁ, Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population. In XV. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita. 2011. ISBN 978-80-210-5594-0.

@proceedings{956114,
   author = {Linhartová, Petra and Řehka, Václav and Bartáková, Vendula and Fassmann, Antonín and Izakovičová Hollá, Lydie},
   booktitle = {XV. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita},
   isbn = {978-80-210-5594-0},
   title = {Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population},
   year = {2011}
}

TY  - CONF
ID  - 956114
AU  - Linhartová, Petra - Řehka, Václav - Bartáková, Vendula - Fassmann, Antonín - Izakovičová Hollá, Lydie
PY  - 2011
TI  - Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population
SN  - 9788021055940
N2  - Periodontitis is an inflammatory disease induced by colonization of the dentogingival interface with pathogenic bacteria and characterized by the connective tissue loss and alveolar bone destruction. Interleukin-8 (IL-8), a CXC chemokine with multiple biological functions, is an important inflammatory mediator responsible for the migration and activation of neutrophils. IL-8 is involved in the initiation and amplification of acute inflammatory reactions and chronic inflammatory processes. The aim of this study was to associate the two single nucleotide polymorphisms in IL-8 with periodontitis and subgingival bacterial colonization in the Czech population. In this case-control association study, a total of 489 unrelated Czech subjects were included. Genomic DNA of 270 patients with chronic periodontitis (CP), 64 patients with aggressive periodontitis (AgP) and 155 healthy/non-periodontitis controls was genotyped using Real-time PCR method for IL-8 gene polymorphisms (RS4073 -251 T/A and RS2227307 +396 T/G). Subgingival bacterial colonization (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia, Treponema denticola, Peptostreptococcus micros, Fusobacterium nucleatum in subgingival pockets) was investigated by the DNA-microarray based on a periodontal pathogen detection kit in a subgroup of subjects (N=169). Allele and genotype frequencies of both investigated polymorphisms were not significantly different between the subjects with CP and/or AgP and controls (P>0.05). However, F. nucleatum occurred less frequently in non-periodontitis individuals positive for the T allele of IL-8 +396 T/G variant (OR = 0.10, confidence interval (CI): 0.01-0.82, P<0.01). In contrast, IL-8 -251 T and +396 T allele carriers had an increased odds ratio (OR) for the individual presence of A. actinomycetemcomitans (previously Actinobacillus actinomycetemcomitans) in AgP patients (OR = 17.0, confidence interval (CI): 1.85-156.27, P<0.01 for -251 T/A and OR = 10.11, confidence interval (CI): 1.05-97.01, P<0.05 for +396 T/G). Although the IL-8 variants could be shown to be associated with subgingival colonization with A. actinomycetemcomitans in AgP, our findings indicate that these polymorphisms alone are not associated with susceptibility to CP or AgP in the Czech population. This study was supported by projects 1M0528 and IGA NT11405-6.
ER  -

LINHARTOVÁ, Petra, Václav ŘEHKA, Vendula BARTÁKOVÁ, Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Association of Interleukin-8 Gene Polymorphisms with Periodontitis and Subgingival Bacterial Colonization in the Czech Population. In \textit{XV. Setkání biochemiků a molekulárních biologů. Brno: Masarykova univerzita}. 2011. ISBN~978-80-210-5594-0.

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