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@proceedings{957085,
author = {Bednařík, Antonín and Ertl, Lukáš and Rážová, Iva and Tomalová, Iva and Preisler, Jan and Moskovets, Eugene},
booktitle = {Colloquium Spectroscopicum Internationale – CSI XXXVII},
keywords = {mass spectrometry imaging; matrix deposition; spincoating; electrospray; piezo actuated spotting},
language = {eng},
isbn = {978-85-8006-046-1},
title = {Investigation of matrix deposition techniques for high throughput MALDI Mass Spectrometry Imaging},
year = {2011}
}
TY - CONF
ID - 957085
AU - Bednařík, Antonín - Ertl, Lukáš - Rážová, Iva - Tomalová, Iva - Preisler, Jan - Moskovets, Eugene
PY - 2011
TI - Investigation of matrix deposition techniques for high throughput MALDI Mass Spectrometry Imaging
SN - 9788580060461
KW - mass spectrometry imaging
KW - matrix deposition
KW - spincoating
KW - electrospray
KW - piezo actuated spotting
N2 - Matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) is an emerging technique with an immense biochemical and pharmaceutical potential, which possesses ability to show spatial distribution of specific compounds in tissues. The crucial step of MALDI-MSI experiment is matrix deposition. The MALDI matrix together with rhodamine B labeled angiotensin I was deposited on sample MALDI plate by three different approaches. By electrospray, spin-coating and piezoelectric nano-spotter. The homogeneity of deposited samples was studied under microscope either by observing simple optical image or in the case of piezo-deposited nano-spots by observing fluorescence image after irradiating the sample with 532 nm laser. Optical image was compared with the MSI generated map of peptide distribution along the samples. To measure spatial distribution of selected peptides, a laboratory-built high-throughput axial time-of-flight (TOF) mass spectrometer with 2 kHz 355 nm Nd:YAG laser was used.
ER -
BEDNAŘÍK, Antonín, Lukáš ERTL, Iva RÁŽOVÁ, Iva TOMALOVÁ, Jan PREISLER a Eugene MOSKOVETS. Investigation of matrix deposition techniques for high throughput MALDI Mass Spectrometry Imaging. In \textit{Colloquium Spectroscopicum Internationale – CSI XXXVII}. 2011. ISBN~978-85-8006-046-1.
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