2011
Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping
ŠVEC, Pavel, Ondrej ŠEDO, Andrea TESHIM, Vladimír DRÁB, Zbyněk ZDRÁHAL et. al.Základní údaje
Originální název
Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping
Autoři
ŠVEC, Pavel (203 Česká republika, garant, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Andrea TESHIM (203 Česká republika, domácí), Vladimír DRÁB (203 Česká republika), Zbyněk ZDRÁHAL (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)
Vydání
4th Congress of European Microbiologists FEMS 2011, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00054000
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova česky
Bifodobacterium; taxonomie; identifikace
Klíčová slova anglicky
Bifodobacterium; taxonomy; identification
Změněno: 5. 1. 2012 11:23, prof. RNDr. Ivo Sedláček, CSc.
Anotace
V originále
Background: Bifidobacteria represent an important part of human gastrointestinal tract maintaining and balancing proper intestinal microflora. Their correct identification is crucial for taxonomical, epidemiological and clinical purposes. Objectives: The aim of this work was to evaluate capabilities of automated ribotyping for identification of bifidobacterial isolates from human intestine. Methods: A set of 70 Bifidobacterium strains originating from intestinal mucous tissue samples from children patients was identified by MALDI-TOF MS performed on an Ultraflex III instrument (Bruker Daltonik) and further characterized using automated ribotyping performed by the RiboPrinter system (DuPont Qualicon). Results: MALDI-TOF MS analysis enabled reliable identification (BioTyper log(score) > 2.3) of Bifidobacterium longum (36 strains), Bifidobacterium adolescentis (23), Bifidobacterium catenulatum (7), Bifidobacterium breve (3) and Bifidobacterium animalis (1). The RiboPrinter system assigned only 33 strains to the species level and identified B. longum (18 strains), B. adolescentis (10), B. breve (3) and single representatives of B. animalis and Bifidobacterium boum. These outcomes were congruent with MALDI-TOF MS results except for two B. adolescentis strains which were identified by ribotyping as B. longum and B. boum, respectively, and six B. catenulatum strains assigned as B. adolescentis. The remaining isolates were not identified as they did not exceed the threshold of 85 % similarity with the RiboPrinter reference database entries. Conclusions: The RiboPrinter system, which identified correctly only 35 % of analysed strains, has been shown as an unsuitable tool for identification of Bifidobacterium spp. occurring in human intestines. This work was supported by projects 2B08068, MSM0021622415, MSM0021622416, and LC06034.
Návaznosti
| LC06034, projekt VaV |
| ||
| MSM0021622415, záměr |
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| MSM0021622416, záměr |
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| 2B08068, projekt VaV |
|