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@proceedings{958881,
author = {Švec, Pavel and Šedo, Ondrej and Teshim, Andrea and Dráb, Vladimír and Zdráhal, Zbyněk and Sedláček, Ivo},
booktitle = {4th Congress of European Microbiologists FEMS 2011},
keywords = {Bifodobacterium; taxonomy; identification},
language = {eng},
title = {Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping},
year = {2011}
}
TY - CONF
ID - 958881
AU - Švec, Pavel - Šedo, Ondrej - Teshim, Andrea - Dráb, Vladimír - Zdráhal, Zbyněk - Sedláček, Ivo
PY - 2011
TI - Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping
KW - Bifodobacterium
KW - taxonomy
KW - identification
N2 - Background: Bifidobacteria represent an important part of human gastrointestinal tract maintaining and balancing proper intestinal microflora. Their correct identification is crucial for taxonomical, epidemiological and clinical purposes. Objectives: The aim of this work was to evaluate capabilities of automated ribotyping for identification of bifidobacterial isolates from human intestine. Methods: A set of 70 Bifidobacterium strains originating from intestinal mucous tissue samples from children patients was identified by MALDI-TOF MS performed on an Ultraflex III instrument (Bruker Daltonik) and further characterized using automated ribotyping performed by the RiboPrinter system (DuPont Qualicon). Results: MALDI-TOF MS analysis enabled reliable identification (BioTyper log(score) > 2.3) of Bifidobacterium longum (36 strains), Bifidobacterium adolescentis (23), Bifidobacterium catenulatum (7), Bifidobacterium breve (3) and Bifidobacterium animalis (1). The RiboPrinter system assigned only 33 strains to the species level and identified B. longum (18 strains), B. adolescentis (10), B. breve (3) and single representatives of B. animalis and Bifidobacterium boum. These outcomes were congruent with MALDI-TOF MS results except for two B. adolescentis strains which were identified by ribotyping as B. longum and B. boum, respectively, and six B. catenulatum strains assigned as B. adolescentis. The remaining isolates were not identified as they did not exceed the threshold of 85 % similarity with the RiboPrinter reference database entries. Conclusions: The RiboPrinter system, which identified correctly only 35 % of analysed strains, has been shown as an unsuitable tool for identification of Bifidobacterium spp. occurring in human intestines. This work was supported by projects 2B08068, MSM0021622415, MSM0021622416, and LC06034.
ER -
ŠVEC, Pavel, Ondrej ŠEDO, Andrea TESHIM, Vladimír DRÁB, Zbyněk ZDRÁHAL a Ivo SEDLÁČEK. Identification of Bifidobacterium spp. isolated from children intestinal mucous tissue samples by MALDI-TOF MS and automated ribotyping. In \textit{4th Congress of European Microbiologists FEMS 2011}. 2011.
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