2011
Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping
TESHIM, Andrea, Ondrej ŠEDO, Pavel ŠVEC, Vladimír DRÁB, Ivo SEDLÁČEK et. al.Základní údaje
Originální název
Identification of Lactobacillus spp. isolates from children's intestinal tissues by MALDI-TOF MS profiling and automated ribotyping
Autoři
TESHIM, Andrea (203 Česká republika, garant, domácí), Ondrej ŠEDO (203 Česká republika, domácí), Pavel ŠVEC (203 Česká republika, domácí), Vladimír DRÁB (203 Česká republika), Ivo SEDLÁČEK (203 Česká republika, domácí) a Zbyněk ZDRÁHAL (203 Česká republika, domácí)
Vydání
4th Congress of European Microbiologists FEMS 2011, 2011
Další údaje
Jazyk
angličtina
Typ výsledku
Konferenční abstrakt
Obor
10600 1.6 Biological sciences
Stát vydavatele
Česká republika
Utajení
není předmětem státního či obchodního tajemství
Kód RIV
RIV/00216224:14310/11:00054001
Organizační jednotka
Přírodovědecká fakulta
Klíčová slova anglicky
Lactobacillus; taxonomy; identification
Změněno: 5. 1. 2012 11:47, doc. RNDr. Pavel Švec, Ph.D.
Anotace
V originále
Background: In clinical microbiology as well as in dairy industry there is a great need for reliable identification and characterization of lactic acid bacteria. Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI TOF MS) represents a fast, procedurally simple and sensitive chemotaxonomical method with a high discriminatory power. The identification of bacteria is based on characteristic and reproducible peptide/protein fingerprints. Objectives: The aim of this work was to assess MALDI TOF MS profiling and automatic ribotyping as tools for reliable identification of Lactobacillus isolates from human intestinal tissues. Methods: MALDI-TOF mass spectra of 57 Lactobacillus strains were obtained with an Ultraflex III instrument (Bruker Daltonik). BioTyper software (Bruker Daltonik) involving a database of lactic acid bacteria MALDI-TOF MS profiles was used for data processing. Automated ribotyping was performed with a RiboPrinter identification system (DuPont Qualicon) including DuPont Qualicon database DUP 2008 provided by the manufacturer. Conclusions: Based on MALDI-TOF MS analysis, Lactobacillus salivarius (28 strains), Lactobacillus paracasei (11), Lactobacillus rhamnosus (7), Lactobacillus mucosae (5), Lactobacillus gasseri (2), Lactobacillus plantarum (2), Lactobacillus casei/paracasiei (1) and Lactobacillus oris (1) were reliably identified (BioTyper log(score) > 2.3). In accordance with MALDI-TOF MS, the RiboPrinter system identified Lactobacillus salivarius (19 strains), Lactobacillus paracasei (12) and Lactobacillus gasseri (1). One L. gasseri strain was assigned as Aerococcus viridans. The remaining strains were not identified using the ribotyping method. MALDI-TOF MS proved to be suitable tool for identification of Lactobacillus spp. inhabiting human intestinal tract. This work was supported by projects 2B08068, MSM0021622415, MSM0021622416 and LC06034.
Návaznosti
| LC06034, projekt VaV |
| ||
| MSM0021622415, záměr |
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| MSM0021622416, záměr |
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| 2B08068, projekt VaV |
|