| VAŘECHA, Miroslav, Michaela POTĚŠILOVÁ, Pavel MATULA a Michal KOZUBEK. Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis. Mol Cell Biochem. 2012, roč. 363, 1-2, s. 301-307. ISSN 0300-8177. Dostupné z: https://dx.doi.org/10.1007/s11010-011-1182-x. |
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@article{959855,
author = {Vařecha, Miroslav and Potěšilová, Michaela and Matula, Pavel and Kozubek, Michal},
article_number = {1-2},
doi = {http://dx.doi.org/10.1007/s11010-011-1182-x},
keywords = {endonuclease G; DNA topoisomerase II alpha; histone H2B; FRET; apoptosis},
language = {eng},
issn = {0300-8177},
journal = {Mol Cell Biochem},
title = {Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis},
url = {http://link.springer.com/article/10.1007%2Fs11010-011-1182-x},
volume = {363},
year = {2012}
}
TY - JOUR
ID - 959855
AU - Vařecha, Miroslav - Potěšilová, Michaela - Matula, Pavel - Kozubek, Michal
PY - 2012
TI - Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis
JF - Mol Cell Biochem
VL - 363
IS - 1-2
SP - 301-307
EP - 301-307
SN - 03008177
KW - endonuclease G
KW - DNA topoisomerase II alpha
KW - histone H2B
KW - FRET
KW - apoptosis
UR - http://link.springer.com/article/10.1007%2Fs11010-011-1182-x
L2 - http://link.springer.com/article/10.1007%2Fs11010-011-1182-x
N2 - During some apoptotic pathways, proteins apoptosis-inducing factor (AIF) and endonuclease G (EndoG) are released from the mitochondria and they translocate into the cell nuclei, where they probably participate in chromatin degradation together with other nuclear proteins. Exact mechanism of EndoG activity in cell nucleus is still unknown. We conducted a living-cell confocal fluorescence microscopy followed by an image analysis of fluorescence resonance energy transfer (FRET) to analyze the possibility of protein interactions of EndoG with histone H2B and human DNA topoisomerase II alpha (TOPO2a). Our results show that EndoG interacts with both these proteins during apoptotic cell death. Therefore, we can conclude that EndoG and TOPO2a may actively participate in apoptotic chromatin degradation. The possible existence of a degradation complex consisting of EndoG and TOPO2a and possibly other proteins like AIF and cyclophilin A have yet to be investigated.
ER -
VAŘECHA, Miroslav, Michaela POTĚŠILOVÁ, Pavel MATULA a Michal KOZUBEK. Endonuclease G interacts with histone H2B and DNA topoisomerase II alpha during apoptosis. \textit{Mol Cell Biochem}. 2012, roč.~363, 1-2, s.~301-307. ISSN~0300-8177. Dostupné z: https://dx.doi.org/10.1007/s11010-011-1182-x.
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