Specificity of the Arabidopsis Shoot and Root Response to
Cytokinins

a 2011

Specificity of the Arabidopsis Shoot and Root Response to Cytokinins

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK et. al.

Základní údaje

Originální název

Specificity of the Arabidopsis Shoot and Root Response to Cytokinins

Autoři

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK, Bedrich PESEK, Eva MIKLÁNKOVÁ, Jana VAŠÍČKOVÁ, Zbyněk ZDRÁHAL a Jan HEJÁTKO

Vydání

5th Central and Eastern European Proteomic Conference, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

5th Central and Eastern European Proteomic Conference in Prague, Czech Republic

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Arabidopsis, cytokinin, ethylene, proteomic analysis, hormonal crosstalk

Příznaky

Mezinárodní význam

Změněno: 30. 11. 2011 13:11, Mgr. Markéta Žďárská, Ph.D.

Anotace

V originále

Cytokinins (CK) have been implicated in regulation of many aspects of plant growth and development. Recent genetic studies suggest that CK play distinct and sometimes even opposite roles in the regulation of the shoot and root development in Arabidopsis. Here we present results of a comprehensive approach in the study of the differential shoot and root CK response. To analyze CK response six days old seedlings of Arabidopsis thaliana (Col-0) were treated with 5 uM CK N6-benzylaminopurine BAP and root and shoot tissues were analyzed separately on diverse levels. Interestingly we identified proteins associated with abscisic acid (ABA)-dependent regulations in shoots, while in roots we detected proteins linked with ethylene metabolism and signalling. To further substantiate the potential crosstalk of BAP with metabolism of other hormones, we have analyzed changes of the endogenous hormone levels after CK application. We observed strong tissue specificity caused by BAP application in endogenous levels of ABA and ACC, the rate-limiting precursor of the ethylene biosynthesis that corresponded to our results of the proteomic analysis.

Návaznosti

LC06034, projekt VaV

Název: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Regulace morfogeneze rostlinných buněk a orgánů

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK, Bedrich PESEK, Eva MIKLÁNKOVÁ, Jana VAŠÍČKOVÁ, Zbyněk ZDRÁHAL a Jan HEJÁTKO. Specificity of the Arabidopsis Shoot and Root Response to Cytokinins. In 5th Central and Eastern European Proteomic Conference. 2011.

@proceedings{960081,
   author = {Žďárská, Markéta and Zatloukalová, Pavlína and Potěšil, David and Šedo, Ondrej and Novak, Ondrej and Pesek, Bedrich and Miklánková, Eva and Vašíčková, Jana and Zdráhal, Zbyněk and Hejátko, Jan},
   booktitle = {5th Central and Eastern European Proteomic Conference},
   keywords = {Arabidopsis, cytokinin, ethylene, proteomic analysis, hormonal crosstalk},
   language = {eng},
   title = {Specificity of the Arabidopsis Shoot and Root Response to Cytokinins},
   url = {http://2011.czproteo.cz/},
   year = {2011}
}

TY  - CONF
ID  - 960081
AU  - Žďárská, Markéta - Zatloukalová, Pavlína - Potěšil, David - Šedo, Ondrej - Novak, Ondrej - Pesek, Bedrich - Miklánková, Eva - Vašíčková, Jana - Zdráhal, Zbyněk - Hejátko, Jan
PY  - 2011
TI  - Specificity of the Arabidopsis Shoot and Root Response to Cytokinins
KW  - Arabidopsis, cytokinin, ethylene, proteomic analysis, hormonal crosstalk
UR  - http://2011.czproteo.cz/
N2  - Cytokinins (CK) have been implicated in regulation of many aspects of plant growth and development. Recent genetic studies suggest that CK play distinct and sometimes even opposite roles in the regulation of the shoot and root development in Arabidopsis. Here we present results of a comprehensive approach in the study of the differential shoot and root CK response. To analyze CK response six days old seedlings of Arabidopsis thaliana (Col-0) were treated with 5 uM CK N6-benzylaminopurine BAP and root and shoot tissues were analyzed separately on diverse levels. Interestingly we identified proteins associated with abscisic acid (ABA)-dependent regulations in shoots, while in roots we detected proteins linked with ethylene metabolism and signalling. To further substantiate the potential crosstalk of BAP with metabolism of other hormones, we have analyzed changes of the endogenous hormone levels after CK application. We observed strong tissue specificity caused by BAP application in endogenous levels of ABA and ACC, the rate-limiting precursor of the ethylene biosynthesis that corresponded to our results of the proteomic analysis.
ER  -

ŽĎÁRSKÁ, Markéta, Pavlína ZATLOUKALOVÁ, David POTĚŠIL, Ondrej ŠEDO, Ondrej NOVAK, Bedrich PESEK, Eva MIKLÁNKOVÁ, Jana VAŠÍČKOVÁ, Zbyněk ZDRÁHAL a Jan HEJÁTKO. Specificity of the Arabidopsis Shoot and Root Response to Cytokinins. In \textit{5th Central and Eastern European Proteomic Conference}. 2011.

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