Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat
Heart Electrogram

J 2011

Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Oto JANOUŠEK, Marina RONZHINA, Ivo PROVAZNÍK et. al.

Základní údaje

Originální název

Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram

Název česky

Účinky napěťově citlivého barviva di-4-ANEPPS u modelu izolovaného srdce potkana

Autoři

FIALOVÁ, Kateřina (203 Česká republika, garant, domácí), Jana KOLÁŘOVÁ (203 Česká republika), Oto JANOUŠEK (203 Česká republika), Marina RONZHINA (203 Česká republika), Ivo PROVAZNÍK (203 Česká republika, domácí) a Marie NOVÁKOVÁ (203 Česká republika, domácí)

Vydání

Computing in Cardiology, China, College of Biomedical Engineering & Instrument Science of Zhejiang University, 2011, 0276-6574

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30201 Cardiac and Cardiovascular systems

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14110/11:00050213

Organizační jednotka

Lékařská fakulta

UT WoS

000305132500182

Klíčová slova anglicky

voltage-sensitive dye;isolated heart; rat;electrogram

Příznaky

Mezinárodní význam

Změněno: 11. 2. 2012 00:22, Mgr. Michal Petr

Anotace

V originále

Although the classical suction microelectrodes are still considered golden standard for recording of monophasic action potentials, there are various new methods enabling to record electrical changes from small area on the heart surface. One of them is recording of cardiac electrical activity using voltage-sensitive dyes (VSDs) by optical method. VSDs exhibit large fluorescence and/or absorption changes that vary with changes of the membrane potential. Staining of the heart with the dye should not result in pharmacological or toxic effects to the preparation. Data about response of the heart to staining with VSD are scarce. In order to determine this response in classical biomodel in basic cardiology, we followed arrythmia-preceding parameters in isolated rat heart electrogram during staining with VSD di-4-ANEPPS and washout periods.

Návaznosti

GA102/07/1473, projekt VaV

Název: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

Investor: Grantová agentura ČR, Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací

MSM0021622402, záměr

Název: Časná diagnostika a léčba kardiovaskulárních chorob

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Časná diagnostika a léčba kardiovaskulárních chorob

Citovat

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Oto JANOUŠEK, Marina RONZHINA, Ivo PROVAZNÍK a Marie NOVÁKOVÁ. Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram. Computing in Cardiology. China: College of Biomedical Engineering & Instrument Science of Zhejiang University, 2011, roč. 38, č. 1, s. 721-724. ISSN 0276-6574.

@article{961152,
   author = {Fialová, Kateřina and Kolářová, Jana and Janoušek, Oto and Ronzhina, Marina and Provazník, Ivo and Nováková, Marie},
   article_location = {China},
   article_number = {1},
   keywords = {voltage-sensitive dye;isolated heart; rat;electrogram},
   language = {eng},
   issn = {0276-6574},
   journal = {Computing in Cardiology},
   note = {sborník},
   title = {Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram},
   url = {http://cinc2011.org},
   volume = {38},
   year = {2011}
}

TY  - JOUR
ID  - 961152
AU  - Fialová, Kateřina - Kolářová, Jana - Janoušek, Oto - Ronzhina, Marina - Provazník, Ivo - Nováková, Marie
PY  - 2011
TI  - Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram
JF  - Computing in Cardiology
VL  - 38
IS  - 1
SP  - 721-724
EP  - 721-724
PB  - College of Biomedical Engineering & Instrument Science of Zhejiang University
SN  - 02766574
N1  - sborník
KW  - voltage-sensitive dye;isolated heart
KW  - rat;electrogram
UR  - http://cinc2011.org
N2  - Although the classical suction microelectrodes are still considered golden standard for recording of monophasic action potentials, there are various new methods enabling to record electrical changes from small area on the heart surface. One of them is recording of cardiac electrical activity using voltage-sensitive dyes (VSDs) by optical method. VSDs exhibit large fluorescence and/or absorption changes that vary with changes of the membrane potential. Staining of the heart with the dye should not result in pharmacological or toxic effects to the preparation. Data about response of the heart to staining with VSD are scarce. In order to determine this response in classical biomodel in basic cardiology, we followed arrythmia-preceding parameters in isolated rat heart electrogram during staining with VSD di-4-ANEPPS and washout periods.
ER  -

FIALOVÁ, Kateřina, Jana KOLÁŘOVÁ, Oto JANOUŠEK, Marina RONZHINA, Ivo PROVAZNÍK a Marie NOVÁKOVÁ. Effects of Voltage-Sensitive Dye di-4-ANEPPS on Isolated Rat Heart Electrogram. \textit{Computing in Cardiology}. China: College of Biomedical Engineering \&{} Instrument Science of Zhejiang University, 2011, roč.~38, č.~1, s.~721-724. ISSN~0276-6574.

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