Genome differences between Treponema pallidum subsp. pallidum
and Treponema pallidum subsp. pertenue.

a 2011

Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue.

ZOBANÍKOVÁ, Marie, Darina ČEJKOVÁ, Michal STROUHAL, Petra POSPÍŠILOVÁ, George M. WEINSTOCK et. al.

Základní údaje

Originální název

Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue.

Autoři

ZOBANÍKOVÁ, Marie, Darina ČEJKOVÁ, Michal STROUHAL, Petra POSPÍŠILOVÁ, George M. WEINSTOCK a David ŠMAJS

Vydání

4th Congress of European Microbiologists FEMS 2011, Geneva, 26.6. – 30.6. 2011, 2011

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

Genetika a molekulární biologie

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Lékařská fakulta

Klíčová slova česky

syfilis; yaws; T. p. pallidum ssp. pallidum; T.p. pallidum ssp. pertenue

Změněno: 17. 1. 2012 13:46, Mgr. Marie Zobaníková, Ph.D.

Anotace

V originále

Background Treponema pallidum ssp. pertenue (TPE) strains, the causative agent of yaws, are closely related to syphilis causing strains of Treponema pallidum ssp. pallidum (TPA). Although clinical symptoms of both diseases differ, bacteria of both subspecies cannot be distinguished by morphology or serology and immunology testing. The whole genome fingerprinting technique identified more than 99% DNA identity between TPA and TPE strains. Objective High quality whole genome sequences of TPE strains are required to precisely define genetic differences between TPA and TPE. Methods Whole genome sequencing methods comprising pyrosequencing (454 Life Sciences), Solexa technology (Illumina), comparative genome sequencing approach (CGS, Nimblegen) and Sanger sequencing were used for sequencing of TPE genomes (SamoaD, CDC-2, Gauthier). Whole genome sequences of TPE strains were compared to sequences of TPA strains (Nichols, Fraser et al. 1998; SS14, Matějková et al. 2007; DAL-1, unpublished). Results A set of 91 annotated TPE proteins (9.3%) contained 2 or more amino acid replacements or other major sequence changes in comparison with TPA proteins. Genes encoding putative virulence factors and hypothetical proteins contained the highest percentage of sequentially divergent proteins when compared to other proteins of known or predicted function. Altogether, 13 pseudogenes were found in the TPE genomes and two TPE genes showed reverted frameshift mutations (TP0009, TP0316). Conclusions Genomic differences between TPE and TPA pathogens were defined. Divergent genes are candidates for virulence determinants of syphilitic treponemes. The specific sequence changes are suitable targets for clinical diagnostics of individual strains.

Návaznosti

GA310/07/0321, projekt VaV

Název: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Grantová agentura ČR, Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Citovat

ZOBANÍKOVÁ, Marie, Darina ČEJKOVÁ, Michal STROUHAL, Petra POSPÍŠILOVÁ, George M. WEINSTOCK a David ŠMAJS. Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue. In 4th Congress of European Microbiologists FEMS 2011, Geneva, 26.6. – 30.6. 2011. 2011.

@proceedings{966726,
   author = {Zobaníková, Marie and Čejková, Darina and Strouhal, Michal and Pospíšilová, Petra and Weinstock, George M. and Šmajs, David},
   booktitle = {4th Congress of European Microbiologists FEMS 2011, Geneva, 26.6. – 30.6. 2011},
   language = {eng},
   title = {Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue.},
   year = {2011}
}

TY  - CONF
ID  - 966726
AU  - Zobaníková, Marie - Čejková, Darina - Strouhal, Michal - Pospíšilová, Petra - Weinstock, George M. - Šmajs, David
PY  - 2011
TI  - Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue.
N2  - Background Treponema pallidum ssp. pertenue (TPE) strains, the causative agent of yaws, are closely related to syphilis causing strains of Treponema pallidum ssp. pallidum (TPA). Although clinical symptoms of both diseases differ, bacteria of both subspecies cannot be distinguished by morphology or serology and immunology testing. The whole genome fingerprinting technique identified more than 99% DNA identity between TPA and TPE strains. Objective High quality whole genome sequences of TPE strains are required to precisely define genetic differences between TPA and TPE. Methods Whole genome sequencing methods comprising pyrosequencing (454 Life Sciences), Solexa technology (Illumina), comparative genome sequencing approach (CGS, Nimblegen) and Sanger sequencing were used for sequencing of TPE genomes (SamoaD, CDC-2, Gauthier). Whole genome sequences of TPE strains were compared to sequences of TPA strains (Nichols, Fraser et al. 1998; SS14, Matějková et al. 2007; DAL-1, unpublished). Results A set of 91 annotated TPE proteins (9.3%) contained 2 or more amino acid replacements or other major sequence changes in comparison with TPA proteins. Genes encoding putative virulence factors and hypothetical proteins contained the highest percentage of sequentially divergent proteins when compared to other proteins of known or predicted function. Altogether, 13 pseudogenes were found in the TPE genomes and two TPE genes showed reverted frameshift mutations (TP0009, TP0316). Conclusions Genomic differences between TPE and TPA pathogens were defined. Divergent genes are candidates for virulence determinants of syphilitic treponemes. The specific sequence changes are suitable targets for clinical diagnostics of individual strains.
ER  -

ZOBANÍKOVÁ, Marie, Darina ČEJKOVÁ, Michal STROUHAL, Petra POSPÍŠILOVÁ, George M. WEINSTOCK a David ŠMAJS. Genome differences between Treponema pallidum subsp. pallidum and Treponema pallidum subsp. pertenue. In \textit{4th Congress of European Microbiologists FEMS 2011, Geneva, 26.6. – 30.6. 2011}. 2011.

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