An improved method for nematode infection assays in Drosophila
larvae

J 2012

An improved method for nematode infection assays in Drosophila larvae

DOBEŠ, Pavel, Zhi WANG, Robert MARKUS, Ulrich THEOPOLD, Pavel HYRŠL et. al.

Základní údaje

Originální název

An improved method for nematode infection assays in Drosophila larvae

Autoři

DOBEŠ, Pavel (203 Česká republika, domácí), Zhi WANG (156 Čína), Robert MARKUS (348 Maďarsko), Ulrich THEOPOLD (752 Švédsko) a Pavel HYRŠL (203 Česká republika, garant, domácí)

Vydání

Fly, Austin (USA), Landes Bioscience, 2012, 1933-6934

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.105

Kód RIV

RIV/00216224:14310/12:00057254

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.4161/fly.19553

UT WoS

000305965500002

Klíčová slova česky

Drosophila melanogaster; Galleria mellonella; entomopatogenní hlístovky; Steinernema feltiae; Heterorhabditis bacteriophora

Klíčová slova anglicky

Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2013 10:52, Ing. Andrea Mikešková

Anotace

V originále

The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.

Návaznosti

GP206/09/P470, projekt VaV

Název: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella

Investor: Grantová agentura ČR, Vliv inhibitorů biosyntézy eikonsanoidů na imunitu zavíječe voskového Galleria mellonella

Citovat

DOBEŠ, Pavel, Zhi WANG, Robert MARKUS, Ulrich THEOPOLD a Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. Fly. Austin (USA): Landes Bioscience, 2012, roč. 6, č. 2, s. 75-79. ISSN 1933-6934. Dostupné z: https://dx.doi.org/10.4161/fly.19553.

@article{969196,
   author = {Dobeš, Pavel and Wang, Zhi and Markus, Robert and Theopold, Ulrich and Hyršl, Pavel},
   article_location = {Austin (USA)},
   article_number = {2},
   doi = {http://dx.doi.org/10.4161/fly.19553},
   keywords = {Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora},
   language = {eng},
   issn = {1933-6934},
   journal = {Fly},
   title = {An improved method for nematode infection assays in Drosophila larvae},
   volume = {6},
   year = {2012}
}

TY  - JOUR
ID  - 969196
AU  - Dobeš, Pavel - Wang, Zhi - Markus, Robert - Theopold, Ulrich - Hyršl, Pavel
PY  - 2012
TI  - An improved method for nematode infection assays in Drosophila larvae
JF  - Fly
VL  - 6
IS  - 2
SP  - 75-79
EP  - 75-79
PB  - Landes Bioscience
SN  - 19336934
KW  - Drosophila melanogaster
KW  - Galleria mellonella
KW  - entomopathogenic nematodes
KW  - Steinernema feltiae
KW  - Heterorhabditis bacteriophora
N2  - The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.
ER  -

DOBEŠ, Pavel, Zhi WANG, Robert MARKUS, Ulrich THEOPOLD a Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. \textit{Fly}. Austin (USA): Landes Bioscience, 2012, roč.~6, č.~2, s.~75-79. ISSN~1933-6934. Dostupné z: https://dx.doi.org/10.4161/fly.19553.

Podrobný výpis o publikaci