An improved method for nematode infection assays in Drosophila
larvae

J 2012

An improved method for nematode infection assays in Drosophila larvae

DOBEŠ, Pavel; Zhi WANG; Robert MARKUS; Ulrich THEOPOLD; Pavel HYRŠL et al.

Basic information

Original name

An improved method for nematode infection assays in Drosophila larvae

Authors

DOBEŠ, Pavel; Zhi WANG; Robert MARKUS; Ulrich THEOPOLD and Pavel HYRŠL

Edition

Fly, Austin (USA), Landes Bioscience, 2012, 1933-6934

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30102 Immunology

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.105

Marked to be transferred to RIV

Yes

RIV identification code

RIV/00216224:14310/12:00057254

Organization unit

Faculty of Science

DOI

https://doi.org/10.4161/fly.19553

UT WoS

000305965500002

Keywords (in Czech)

Drosophila melanogaster; Galleria mellonella; entomopatogenní hlístovky; Steinernema feltiae; Heterorhabditis bacteriophora

Keywords in English

Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora

Abstract

In the original language

The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.

Links

GP206/09/P470, research and development project

Name: Vliv inhibitorů biosyntézy eikosanoidů na imunitu zavíječe voskového Galleria mellonella

Investor: Czech Science Foundation, The influence of the eicosanoid biosynthesis inhibitors to immunity of the wax moth Galleria mellonella

Cite

DOBEŠ, Pavel; Zhi WANG; Robert MARKUS; Ulrich THEOPOLD and Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. Fly. Austin (USA): Landes Bioscience, 2012, vol. 6, No 2, p. 75-79. ISSN 1933-6934. Available from: https://doi.org/10.4161/fly.19553.

@article{969196,
   author = {Dobeš, Pavel and Wang, Zhi and Markus, Robert and Theopold, Ulrich and Hyršl, Pavel},
   article_location = {Austin (USA)},
   article_number = {2},
   doi = {https://doi.org/10.4161/fly.19553},
   keywords = {Drosophila melanogaster; Galleria mellonella; entomopathogenic nematodes; Steinernema feltiae; Heterorhabditis bacteriophora},
   language = {eng},
   issn = {1933-6934},
   journal = {Fly},
   title = {An improved method for nematode infection assays in Drosophila larvae},
   volume = {6},
   year = {2012}
}

TY  - JOUR
ID  - 969196
AU  - Dobeš, Pavel - Wang, Zhi - Markus, Robert - Theopold, Ulrich - Hyršl, Pavel
PY  - 2012
TI  - An improved method for nematode infection assays in Drosophila larvae
JF  - Fly
VL  - 6
IS  - 2
SP  - 75-79
EP  - 75-79
PB  - Landes Bioscience
SN  - 19336934
KW  - Drosophila melanogaster
KW  - Galleria mellonella
KW  - entomopathogenic nematodes
KW  - Steinernema feltiae
KW  - Heterorhabditis bacteriophora
N2  - The infective juveniles (IJs) of entomopathogenic nematodes (EPNs) seek out host insects and release their symbiotic bacteria into their body cavity causing septicaemia, which eventually leads to host death. The interaction between EPNs and their hosts are only partially understood, in particular the host immune responses appears to involve pathways other than phagocytosis and the canonical transcriptional induction pathways. These pathways are genetically tractable and include for example clotting factors and lipid mediators. The aim of this study was to optimize the nematode infections in Drosophila melanogaster larvae, a well-studied and genetically tractable model organism. Here we show that two nematode species namely Steinernema feltiae and Heterorhabditis bacteriophora display different infectivity towards Drosophila larvae with the latter being less pathogenic. The effects of supporting media and IJ dosage on the mortality of the hosts were assessed and optimized. Using optimum conditions, a faster and efficient setup for nematode infections was developed. This newly established infection model in Drosophila larvae will be applicable in large scale screens aimed at identifying novel genes/pathways involved in innate immune responses.
ER  -

DOBEŠ, Pavel; Zhi WANG; Robert MARKUS; Ulrich THEOPOLD and Pavel HYRŠL. An improved method for nematode infection assays in Drosophila larvae. \textit{Fly}. Austin (USA): Landes Bioscience, 2012, vol.~6, No~2, p.~75-79. ISSN~1933-6934. Available from: https://doi.org/10.4161/fly.19553.

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