Léčba kostního můstku transplantací mezenchymových kmenových buněk a chondrocytů v kompozitním nosiči u prasat – experimentální studie

PLÁNKA, Ladislav, A. NEČAS, M. CRHA, P. PROKS, L. VOJTOVÁ a Petr GÁL. Léčba kostního můstku transplantací mezenchymových kmenových buněk a chondrocytů v kompozitním nosiči u prasat – experimentální studie. Acta Chirurgiae Orthopaedicae et Traumatologiae Čechoslovaca. 2011, roč. 78, č. 6, s. 528–536. ISSN 0001-5415.

Základní údaje

• Originální název: Léčba kostního můstku transplantací mezenchymových kmenových buněk a chondrocytů v kompozitním nosiči u prasat – experimentální studie
• Název anglicky: Treatment of a Bone Bridge by Transplantation of Mesenchymal Stem Cells and Chondrocytes in a Composite Scaffold in Pigs. Experimental Study
• Autoři: PLÁNKA, Ladislav (203 Česká republika, garant, domácí), A. NEČAS (203 Česká republika), M. CRHA (203 Česká republika), P. PROKS (203 Česká republika), L. VOJTOVÁ (203 Česká republika) a Petr GÁL (203 Česká republika, domácí).
• Vydání: Acta Chirurgiae Orthopaedicae et Traumatologiae Čechoslovaca, 2011, 0001-5415.

Další údaje

• Originální jazyk: čeština
• Typ výsledku: Článek v odborném periodiku
• Obor: 30200 3.2 Clinical medicine
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Impakt faktor: Impact factor: 1.628 v roce 2009
• Kód RIV: RIV/00216224:14110/11:00055460
• Organizační jednotka: Lékařská fakulta
• UT WoS: 000298771400007
• Klíčová slova anglicky: mesenchymal stem cells; growth plate; bone bridge; scaffold
• Příznaky: Mezinárodní význam

Anotace

Předkládaná experimentální studie popisuje výsledky použití kombinace alogenních mezenchymových kmenových buněk (MSCs) s chondrocyty (CHC) a nového nosiče složeného z kolagenu typu I a chitosanových vláken. Transplantace uvedeného biokompozitu do defektu vzniklého po resekci kostního můstku měla indukovat tvorbu nové chrupavčité tkáně. Transplantace byla provedena do defektu distální epifýzy levé stehenní kosti, pravá sloužila jako kontrola, zde byl implantován samotný nosič. Byl tedy očekáván lepší terapeutický výsledek na levé stehenní kosti – redukce růstové i úhlové deformity a histologický nález odpovídající tvorbě tkáně podobné chrupavce v resekovaném defektu levé stehenní kosti.

Anotace anglicky

The presented experimental study describes the results of using a combination of allogeneic mesenchymal cells (MSCs) with chondrocytes (CHCs) and a novel scaffold based on type I collagen and chitosan fibres. This biocomposite was transplanted into a defect produced by excision of a bone bridge to induce new cartilaginous tissue formation. The left femur was treated by transplantation into a defect of distal epiphysis; the right femur with implantation of the scaffold only served as control. A better therapeutic result was therefore expected in the left femur - the reduction of growth and angular deformities, and the histological' finding of a tissue similar to the cartilage excised from the left femur.. MATERIAL AND METHODS The miniature pig was selected as an experimental model and 10 pigs were used. Mesenchymal stem cells derived from femoral bone marrow and chondrocytes derived from a sample harvested from the non-weight-bearing articular surface of the distal end of the femur were cultured in medium. The novel scaffold was based on collagen containing chitosan nanofibres. To make manipulation during implantation easier, the cilindrical scaffolds after lyophilisation were again placed in 96-well plates for seeding. The scaffolds before implantation were seeded with 2x106 allogeneic MSCs and 1x106 allogeneic CHCs. The outcomes of treatment were assessed by measuring the length of bone and the degree of distal femoral valgus deformity, and by the histological findings obtained (properties and maturity of the newly-formed tissue, detection of type II collagen, PAS reaction). RESULTS The right and left legs were examined for longitudinal bone growth and the valgus angle and compared. The treated left leg showed a higher average value for longitudinal growth than the untreated right leg (p = 0.004). The average degree of angular deformity was lower in the left leg than in the right leg (p = 0.008). The microscopic findings showed that a tissue similar to hyaline cartilage was more frequently present in the femoral bone defect of the left leg, as compared with that of the right leg. Type II collagen was detected more frequently and at higher amounts on the left than the right side (p = 0.033). The PAS reaction was positive in all left limbs, with a high degree of positivity in 80 % of them, while this was not achieved in any of the right limbs (p = 0.001). DISCUSSION The use of stem cells in the indication reported here has only been the matter of time since the information on encouraging results in neurology and cardiology was published. First studies with positive results have soon been reported. The initial hydrogel scaffolds were based on tissue adhesives. However, they were not stable enough and were difficult to handle during surgery. In further studies, therefore, the use was made of a three-dimensional scaffold with a self-supporting structure of collagen fibres. This structure also facilitated its hydrodynamic seeding with MSCs and CHCs, which is an effective and sparing procedure for the transplanted cells. Studies concerned with MSCs and/or CHCs transplantation for repair of a physeal defect following bone bridge excision, i.e. for bone bridge treatment, in a broader experimental design, however, are still missing. CONCLUSION Transplantation of a composite scaffold seeded with mesenchymal stem cells and chondrocytes into a physeal defect following bone bridge excision prevented growth disturbance and angular deformity development in the distal femoral epiphysis. In comparison with the control group, it resulted in a more frequent production of a tissue similar to hyaline cartilage, with a cell formation reminiscent of a typical columnar arrangement of the growth plate.