Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum

CAESAR, Katharina, Antje M. K. THAMM, Janika WITTHÖFT, Kirstin ELGASS, Peter HUPPENBERGER, Christopher GREFEN, Jakub HORÁK and Klaus HARTER. Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2011, vol. 62, No 15, p. 5571-5580. ISSN 0022-0957. Available from: https://dx.doi.org/10.1093/jxb/err238.

Basic information

• Original name: Evidence for the localization of the Arabidopsis cytokinin receptors AHK3 and AHK4 in the endoplasmic reticulum
• Authors: CAESAR, Katharina (276 Germany), Antje M. K. THAMM (276 Germany), Janika WITTHÖFT (276 Germany), Kirstin ELGASS (276 Germany), Peter HUPPENBERGER (276 Germany), Christopher GREFEN (826 United Kingdom of Great Britain and Northern Ireland), Jakub HORÁK (203 Czech Republic, belonging to the institution) and Klaus HARTER (276 Germany, guarantor).
• Edition: Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2011, 0022-0957.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 5.364
• RIV identification code: RIV/00216224:14740/11:00055502
• Organization unit: Central European Institute of Technology
• Doi: http://dx.doi.org/10.1093/jxb/err238
• UT WoS: 000297373500028
• Keywords in English: AHK3; cytokinin perception; endoplasmic reticulum; FIDSAM
• Tags: ok, rivok
• Tags: International impact, Reviewed

Abstract

Cytokinins are hormones that are involved in various processes of plant growth and development. The model of cytokinin signalling starts with hormone perception through membrane-localized histidine kinase receptors. Although the biochemical properties and functions of these receptors have been extensively studied, there is no solid proof of their subcellular localization. Here, cell biological and biochemical evidence for the localization of functional fluorophor-tagged fusions of Arabidopsis histidine kinase 3 (AHK3) and 4 (AHK4), members of the cytokinin receptor family, in the endoplasmic reticulum (ER) is provided. Furthermore, membrane-bound AHK3 interacts with AHK4 in vivo. The ER localization and putative function of cytokinin receptors from the ER have major impacts on the concept of cytokinin perception and signalling, and hormonal cross-talk in plants.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs