Physiological and proteomic approaches to evaluate the role of
sterol binding in elicitin-induced resistance

J 2012

Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance

DOKLÁDAL, Ladislav; Michal OBOŘIL; Karel STEJSKAL; Zbyněk ZDRÁHAL; Nikola PTÁČKOVÁ et al.

Základní údaje

Originální název

Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance

Autoři

Vydání

Journal of Experimental Botany, Oxford, OXFORD UNIV PRESS, 2012, 0022-0957

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.242

Kód RIV

RIV/00216224:14310/12:00057274

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1093/jxb/err427

UT WoS

000301297800031

Klíčová slova anglicky

Elicitins; intercellular proteome; omega-loop conformation; sterol binding; resistance induction

Štítky

AKR, podil, rivok

Změněno: 8. 12. 2015 09:30, prof. Mgr. Tomáš Kašparovský, Ph.D.

Anotace

V originále

Cryptogein is a proteinaceous elicitor secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind sterols, phospholipids or both. The sterol binding and phospholipid transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However, changes in the intercellular proteome corresponded to transcription levels of defence genes and resistance to P. parasitica and structureprediction of mutants did not reveal any significant changes in protein structure. These results suggest, contrary to previous proposals, that the sterol-binding ability of cryptogein and its mutants, and the associated conformational change in the v-loop, might not be principal factors in either ROS production or resistance induction. Nevertheless, the results support the importance of the v-loop for the interaction of the protein with the high affinity binding site on the plasma membrane.

Návaznosti

GAP501/11/1003, projekt VaV

Název: Interakce mutantních forem cryptogeinu s membránovým vazebným místem: charakterizace pomocí biosensorů

Investor: Grantová agentura ČR, Interactions of mutant forms of cryptogein with a membrane binding site: biosensors-based characterisation

GP203/09/P248, projekt VaV

Název: Mechanizmus interakce ergosterolu s rostlinnou buňkou

Investor: Grantová agentura ČR, Mechanizmus interakce ergosterolu s rostlinnou buňkou

MSM0021622413, záměr

Název: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Proteiny v metabolismu a při interakci organismů s prostředím

MSM0021622415, záměr

Název: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Molekulární podstata buněčných a tkáňových regulací

Přiložené soubory

ZVV_2012_06_972643.pdf

Požádat o autorskou verzi souboru

Citovat

DOKLÁDAL, Ladislav; Michal OBOŘIL; Karel STEJSKAL; Zbyněk ZDRÁHAL; Nikola PTÁČKOVÁ; Radka CHALOUPKOVÁ; Jiří DAMBORSKÝ; Tomáš KAŠPAROVSKÝ; Jeandroz SYLVAIN; Markéta ŽĎÁRSKÁ a Jan LOCHMAN. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2012, roč. 63, č. 5, s. 2203-2215. ISSN 0022-0957. Dostupné z: https://doi.org/10.1093/jxb/err427.

@article{972643,
   author = {Dokládal, Ladislav and Obořil, Michal and Stejskal, Karel and Zdráhal, Zbyněk and Ptáčková, Nikola and Chaloupková, Radka and Damborský, Jiří and Kašparovský, Tomáš and Sylvain, Jeandroz and Žďárská, Markéta and Lochman, Jan},
   article_location = {Oxford},
   article_number = {5},
   doi = {https://doi.org/10.1093/jxb/err427},
   keywords = {Elicitins; intercellular proteome; omega-loop conformation; sterol binding; resistance induction},
   language = {eng},
   issn = {0022-0957},
   journal = {Journal of Experimental Botany},
   title = {Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance},
   volume = {63},
   year = {2012}
}

TY  - JOUR
ID  - 972643
AU  - Dokládal, Ladislav - Obořil, Michal - Stejskal, Karel - Zdráhal, Zbyněk - Ptáčková, Nikola - Chaloupková, Radka - Damborský, Jiří - Kašparovský, Tomáš - Sylvain, Jeandroz - Žďárská, Markéta - Lochman, Jan
PY  - 2012
TI  - Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance
JF  - Journal of Experimental Botany
VL  - 63
IS  - 5
SP  - 2203-2215
EP  - 2203-2215
PB  - OXFORD UNIV PRESS
SN  - 00220957
KW  - Elicitins
KW  - intercellular proteome
KW  - omega-loop conformation
KW  - sterol binding
KW  - resistance induction
N2  - Cryptogein is a proteinaceous elicitor secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind sterols, phospholipids or both. The sterol binding and phospholipid transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However, changes in the intercellular proteome corresponded to transcription levels of defence genes and resistance to P. parasitica and structureprediction of mutants did not reveal any significant changes in protein structure. These results suggest, contrary to previous proposals, that the sterol-binding ability of cryptogein and its mutants, and the associated conformational change in the v-loop, might not be principal factors in either ROS production or resistance induction. Nevertheless, the results support the importance of the v-loop for the interaction of the protein with the high affinity binding site on the plasma membrane.
ER  -

DOKLÁDAL, Ladislav; Michal OBOŘIL; Karel STEJSKAL; Zbyněk ZDRÁHAL; Nikola PTÁČKOVÁ; Radka CHALOUPKOVÁ; Jiří DAMBORSKÝ; Tomáš KAŠPAROVSKÝ; Jeandroz SYLVAIN; Markéta ŽĎÁRSKÁ a Jan LOCHMAN. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2012, roč.~63, č.~5, s.~2203-2215. ISSN~0022-0957. Dostupné z: https://doi.org/10.1093/jxb/err427.

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