| DOKLÁDAL, Ladislav, Michal OBOŘIL, Karel STEJSKAL, Zbyněk ZDRÁHAL, Nikola PTÁČKOVÁ, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ, Tomáš KAŠPAROVSKÝ, Jeandroz SYLVAIN, Markéta ŽĎÁRSKÁ a Jan LOCHMAN. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. Journal of Experimental Botany. Oxford: OXFORD UNIV PRESS, 2012, roč. 63, č. 5, s. 2203-2215. ISSN 0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/err427. |
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@article{972643,
author = {Dokládal, Ladislav and Obořil, Michal and Stejskal, Karel and Zdráhal, Zbyněk and Ptáčková, Nikola and Chaloupková, Radka and Damborský, Jiří and Kašparovský, Tomáš and Sylvain, Jeandroz and Žďárská, Markéta and Lochman, Jan},
article_location = {Oxford},
article_number = {5},
doi = {http://dx.doi.org/10.1093/jxb/err427},
keywords = {Elicitins; intercellular proteome; omega-loop conformation; sterol binding; resistance induction},
language = {eng},
issn = {0022-0957},
journal = {Journal of Experimental Botany},
title = {Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance},
volume = {63},
year = {2012}
}
TY - JOUR
ID - 972643
AU - Dokládal, Ladislav - Obořil, Michal - Stejskal, Karel - Zdráhal, Zbyněk - Ptáčková, Nikola - Chaloupková, Radka - Damborský, Jiří - Kašparovský, Tomáš - Sylvain, Jeandroz - Žďárská, Markéta - Lochman, Jan
PY - 2012
TI - Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance
JF - Journal of Experimental Botany
VL - 63
IS - 5
SP - 2203-2215
EP - 2203-2215
PB - OXFORD UNIV PRESS
SN - 00220957
KW - Elicitins
KW - intercellular proteome
KW - omega-loop conformation
KW - sterol binding
KW - resistance induction
N2 - Cryptogein is a proteinaceous elicitor secreted by Phytophthora cryptogea that can induce resistance to P. parasitica in tobacco plants. On the basis of previous computer modelling experiments, by site-directed mutagenesis a series of cryptogein variants was prepared with altered abilities to bind sterols, phospholipids or both. The sterol binding and phospholipid transfer activities corresponded well with the previously reported structural data. Induction of the synthesis of reactive oxygen species (ROS) in tobacco cells in suspension and proteomic analysis of intercellular fluid changes in tobacco leaves triggered by these mutant proteins were not proportional to their ability to bind or transfer sterols and phospholipids. However, changes in the intercellular proteome corresponded to transcription levels of defence genes and resistance to P. parasitica and structureprediction of mutants did not reveal any significant changes in protein structure. These results suggest, contrary to previous proposals, that the sterol-binding ability of cryptogein and its mutants, and the associated conformational change in the v-loop, might not be principal factors in either ROS production or resistance induction. Nevertheless, the results support the importance of the v-loop for the interaction of the protein with the high affinity binding site on the plasma membrane.
ER -
DOKLÁDAL, Ladislav, Michal OBOŘIL, Karel STEJSKAL, Zbyněk ZDRÁHAL, Nikola PTÁČKOVÁ, Radka CHALOUPKOVÁ, Jiří DAMBORSKÝ, Tomáš KAŠPAROVSKÝ, Jeandroz SYLVAIN, Markéta ŽĎÁRSKÁ a Jan LOCHMAN. Physiological and proteomic approaches to evaluate the role of sterol binding in elicitin-induced resistance. \textit{Journal of Experimental Botany}. Oxford: OXFORD UNIV PRESS, 2012, roč.~63, č.~5, s.~2203-2215. ISSN~0022-0957. Dostupné z: https://dx.doi.org/10.1093/jxb/err427.
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