Electrochemical Biosensor for Detection of Bioagents

J 2011

Electrochemical Biosensor for Detection of Bioagents

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL, Petr SKLÁDAL et. al.

Základní údaje

Originální název

Electrochemical Biosensor for Detection of Bioagents

Název česky

Elektrochemický biosensor pro detekci biologických látek

Autoři

ŠVÁBENSKÁ, Eva (203 Česká republika, domácí), David KOVÁŘ (203 Česká republika, domácí), Vít KRAJÍČEK (203 Česká republika, domácí), Jan PŘIBYL (203 Česká republika, domácí) a Petr SKLÁDAL (203 Česká republika, garant, domácí)

Vydání

International Journal of Electrochemical Science, Belgrade, Serbia, ESG, 2011, 1452-3981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.729

Kód RIV

RIV/00216224:14310/11:00055984

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000297571400002

Klíčová slova česky

amerometrický sensor; sítotiskové elektrody; imunochemický detektor; imunosensor

Klíčová slova anglicky

amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor

Štítky

AKR, rivok

Změněno: 16. 4. 2012 09:23, Ing. Andrea Mikešková

Anotace

V originále

Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials.

Návaznosti

LC06030, projekt VaV

Název: Biomolekulární centrum

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Biomolekulární centrum

Citovat

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL a Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. International Journal of Electrochemical Science. Belgrade, Serbia: ESG, 2011, roč. 6, č. 12, s. 5968-5979. ISSN 1452-3981.

@article{974916,
   author = {Švábenská, Eva and Kovář, David and Krajíček, Vít and Přibyl, Jan and Skládal, Petr},
   article_location = {Belgrade, Serbia},
   article_number = {12},
   keywords = {amperometric sensor; screen-printed electrode; immunochemical detector; immunosensor},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Electrochemical Biosensor for Detection of Bioagents},
   url = {http://www.electrochemsci.org/papers/vol6/6125968.pdf},
   volume = {6},
   year = {2011}
}

TY  - JOUR
ID  - 974916
AU  - Švábenská, Eva - Kovář, David - Krajíček, Vít - Přibyl, Jan - Skládal, Petr
PY  - 2011
TI  - Electrochemical Biosensor for Detection of Bioagents
JF  - International Journal of Electrochemical Science
VL  - 6
IS  - 12
SP  - 5968-5979
EP  - 5968-5979
PB  - ESG
SN  - 14523981
KW  - amperometric sensor
KW  - screen-printed electrode
KW  - immunochemical detector
KW  - immunosensor
UR  - http://www.electrochemsci.org/papers/vol6/6125968.pdf
L2  - http://www.electrochemsci.org/papers/vol6/6125968.pdf
N2  - Simple and rapid detection and identification of dangerous bioagents is important for preventing illness or even death of people due to infectious diseases and bioterrorist threats. Electrochemical detection has a large potential for development of portable instrumentation suitable for analysis in field and especially combination with specificity of immunoassays seems promising. Initially, the construction of the immunosensing layer was realised and the effect of Au nanoparticles on the response of peroxidase was studied. An amperometric immunosensor was developed using screen-printed transducer, it employed specific capture of microbes in the sensing area by formation of an immunocomplex and its subsequent labelling using the tracer - antibody conjugated to peroxidase. The obtained sandwich electrochemical immunoassay was applied for the model microbial cells of B. subtilis. The total time of analysis was 20 min and the detection of cells below 104 CFU/ml was feasible. The presence of microbial cells at the sensing surface was confirmed using atomic force microscopy. The results appear promising for combination of the immunosensor with a cyclone sampler and analysis of Bacillus-based bioaerosols in field trials.
ER  -

ŠVÁBENSKÁ, Eva, David KOVÁŘ, Vít KRAJÍČEK, Jan PŘIBYL a Petr SKLÁDAL. Electrochemical Biosensor for Detection of Bioagents. \textit{International Journal of Electrochemical Science}. Belgrade, Serbia: ESG, 2011, roč.~6, č.~12, s.~5968-5979. ISSN~1452-3981.

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