| KONEČNÁ, Hana, Lukáš MULLER, Hana DOSOUDILOVÁ, David POTĚŠIL, Jana BURŠÍKOVÁ, Ondrej ŠEDO, Ivana MÁROVÁ a Zbyněk ZDRÁHAL. Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients. J. Agric. Food Chem. WASHINGTON: American Chemical Society, 2012, roč. 60, č. 10, s. 2418-2426. ISSN 0021-8561. Dostupné z: https://dx.doi.org/10.1021/jf204475e. |
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@article{978531,
author = {Konečná, Hana and Muller, Lukáš and Dosoudilová, Hana and Potěšil, David and Buršíková, Jana and Šedo, Ondrej and Márová, Ivana and Zdráhal, Zbyněk},
article_location = {WASHINGTON},
article_number = {10},
doi = {http://dx.doi.org/10.1021/jf204475e},
keywords = {beer proteome; two-dimensional gel electrophoresis; OFFGEL prefractionation; mass spectrometry; Hordeum vulgare; Saccharomyces species},
language = {eng},
issn = {0021-8561},
journal = {J. Agric. Food Chem.},
title = {Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients},
url = {http://pubs.acs.org/doi/abs/10.1021/jf204475e},
volume = {60},
year = {2012}
}
TY - JOUR
ID - 978531
AU - Konečná, Hana - Muller, Lukáš - Dosoudilová, Hana - Potěšil, David - Buršíková, Jana - Šedo, Ondrej - Márová, Ivana - Zdráhal, Zbyněk
PY - 2012
TI - Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients
JF - J. Agric. Food Chem.
VL - 60
IS - 10
SP - 2418-2426
EP - 2418-2426
PB - American Chemical Society
SN - 00218561
KW - beer proteome
KW - two-dimensional gel electrophoresis
KW - OFFGEL prefractionation
KW - mass spectrometry
KW - Hordeum vulgare
KW - Saccharomyces species
UR - http://pubs.acs.org/doi/abs/10.1021/jf204475e
L2 - http://pubs.acs.org/doi/abs/10.1021/jf204475e
N2 - Two-dimensional gel electrophoresis in combination with mass spectrometry has already been applied successfully to study beer proteome. Due to the abundance of protein Z in beer samples, prefractionation techniques might help to improve beer proteome coverage. Proteins from four lager beers of different origins were separated by two-dimensional electrophoresis (2-DE) followed by tandem mass spectrometric analysis. Initially 52 proteins mostly from Hordeum vulgare (22 proteins) and Saccharomyces species (25 proteins) were identified. Preparative isoelectric focusing by OFFGEL Fractionator was applied prior to 2-DE to improve its resolution power. As a result of this combined approach, a total of 70 beer proteins from Hordeum vulgare (30 proteins), from Saccharomyces species (31 proteins), and from other sources (9 proteins) were identified. Of these, 37 proteins have not been previously reported in beer samples.
ER -
KONEČNÁ, Hana, Lukáš MULLER, Hana DOSOUDILOVÁ, David POTĚŠIL, Jana BURŠÍKOVÁ, Ondrej ŠEDO, Ivana MÁROVÁ a Zbyněk ZDRÁHAL. Exploration of Beer Proteome Using OFFGEL Prefractionation in Combination with Two-Dimensional Gel Electrophoresis with Narrow pH Range Gradients. \textit{J. Agric. Food Chem.}. WASHINGTON: American Chemical Society, 2012, roč.~60, č.~10, s.~2418-2426. ISSN~0021-8561. Dostupné z: https://dx.doi.org/10.1021/jf204475e.
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