Electrochemical Immunosensor Coupled to Cyclone Air Sampler for
Detection of Escherichia coli DH5 alpha in Bioaerosols

J 2012

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols

SKLÁDAL, Petr, Eva ŠVÁBENSKÁ, Jiří ŽERAVÍK, Jan PŘIBYL, Petra ŠIŠKOVÁ et. al.

Basic information

Original name

Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols

Authors

SKLÁDAL, Petr (203 Czech Republic, guarantor, belonging to the institution), Eva ŠVÁBENSKÁ (203 Czech Republic, belonging to the institution), Jiří ŽERAVÍK (203 Czech Republic, belonging to the institution), Jan PŘIBYL (203 Czech Republic, belonging to the institution), Petra ŠIŠKOVÁ (203 Czech Republic, belonging to the institution), Torbjorn TJARNHAGE (752 Sweden) and Inga GUSTAFSON (752 Sweden)

Edition

Electroanalysis, WILEY-VCH Verlag GmbH, 2012, 1040-0397

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.817

RIV identification code

RIV/00216224:14310/12:00059725

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/elan.201100448

UT WoS

000300931000011

Keywords (in Czech)

bioagen; sendvičové imunostanovení; biosensor; vzdálené měření; aerosolová komora

Keywords in English

Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber

Abstract

ORIG CZ

V originále

The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.

In Czech

Přenosný imunosensor ImmunoSMART byl ve spojení s cyklonem použit pro imunochemické stanovení bioaerosolu z Escherichia coli DH5alfa v aerosolové komoře.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

SKLÁDAL, Petr, Eva ŠVÁBENSKÁ, Jiří ŽERAVÍK, Jan PŘIBYL, Petra ŠIŠKOVÁ, Torbjorn TJARNHAGE and Inga GUSTAFSON. Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols. Electroanalysis. WILEY-VCH Verlag GmbH, 2012, vol. 24, No 3, p. 539-546. ISSN 1040-0397. Available from: https://dx.doi.org/10.1002/elan.201100448.

@article{979582,
   author = {Skládal, Petr and Švábenská, Eva and Žeravík, Jiří and Přibyl, Jan and Šišková, Petra and Tjarnhage, Torbjorn and Gustafson, Inga},
   article_number = {3},
   doi = {http://dx.doi.org/10.1002/elan.201100448},
   keywords = {Bioagents; Sandwich immunoassay; Biosensors; Remote sensing; Aerosol chamber},
   language = {eng},
   issn = {1040-0397},
   journal = {Electroanalysis},
   title = {Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols},
   volume = {24},
   year = {2012}
}

TY  - JOUR
ID  - 979582
AU  - Skládal, Petr - Švábenská, Eva - Žeravík, Jiří - Přibyl, Jan - Šišková, Petra - Tjarnhage, Torbjorn - Gustafson, Inga
PY  - 2012
TI  - Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols
JF  - Electroanalysis
VL  - 24
IS  - 3
SP  - 539-546
EP  - 539-546
PB  - WILEY-VCH Verlag GmbH
SN  - 10400397
KW  - Bioagents
KW  - Sandwich immunoassay
KW  - Biosensors
KW  - Remote sensing
KW  - Aerosol chamber
N2  - The portable detector ImmunoSMART was combined with the cyclone air sampler to detect the model bioagent E. coli DH5a in bioaerosols. The selected capture antibody was immobilized on gold electrodes, antibody-peroxidase conjugate served as a tracer. In buffer, the amperometric immunosensor measured 103 to 108 CFU/mL within 30 min. Detection in air was realized in a closed aerosol chamber. Automated dissemination of the cells, sampling and measurement allowed remote testing of the cyclone/immunodetector. The level of 150 CFU/L in air was clearly indicated (20 min analysis including 5 min sampling) and independently confirmed using the slit sampler and agar cultivation. The results seem promising for further extended tests in both aerosol chamber and field trial conditions.
ER  -

SKLÁDAL, Petr, Eva ŠVÁBENSKÁ, Jiří ŽERAVÍK, Jan PŘIBYL, Petra ŠIŠKOVÁ, Torbjorn TJARNHAGE and Inga GUSTAFSON. Electrochemical Immunosensor Coupled to Cyclone Air Sampler for Detection of Escherichia coli DH5 alpha in Bioaerosols. \textit{Electroanalysis}. WILEY-VCH Verlag GmbH, 2012, vol.~24, No~3, p.~539-546. ISSN~1040-0397. Available from: https://dx.doi.org/10.1002/elan.201100448.

Detailed Information on Publication Record