Detailed Information on Publication Record
2012
Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.
LENGEROVÁ, Martina, Iva KOCMANOVÁ, Zdeněk RÁČIL, Kristýna HRNČÍŘOVÁ, Šárka POSPÍŠILOVÁ et. al.Basic information
Original name
Detection and measurement of fungal burden in a guinea pig model of invasive pulmonary aspergillosis by novel quantitative nested real-time PCR compared with galactomannan and (1,3)-beta-D-glucan detection.
Authors
LENGEROVÁ, Martina (203 Czech Republic, guarantor, belonging to the institution), Iva KOCMANOVÁ (203 Czech Republic, belonging to the institution), Zdeněk RÁČIL (203 Czech Republic, belonging to the institution), Kristýna HRNČÍŘOVÁ (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Jiří MAYER (203 Czech Republic, belonging to the institution), Laura K NAJVAR (840 United States of America), Nathan P WIEDERHOLD (840 United States of America), William R KIRKPATRICK (840 United States of America) and Thomas F PATTERSON (840 United States of America)
Edition
Journal of Clinical Microbiology, Washington, American Society for Microbiology, 2012, 0095-1137
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30200 3.2 Clinical medicine
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 4.068
RIV identification code
RIV/00216224:14740/12:00058696
Organization unit
Central European Institute of Technology
UT WoS
000300997800011
Keywords in English
Invasive pulmonary aspergillosis; real-time PCR; galactomannan; glucan
Tags
International impact, Reviewed
Změněno: 10/4/2013 08:27, Olga Křížová
Abstract
V originále
We developed and assessed the diagnostic value of a novel quantitative nested real-time (QNRT) PCR assay targeting the internal transcribed spacer region of ribosomal DNA (rDNA) in a guinea pig model of invasive pulmonary aspergillosis. Groups of 5 immunosuppressed animals that were infected using an aerosol chamber with Aspergillus fumigatus conidia were humanely terminated 1 h postinoculation and at days 3,5,7,and 11 postchallenge, and lung tissue, bronchoalveolar lavage (BAL) fluid, whole blood, and serum samples were collected. The QNRT PCR results obtained with the serum and BAL fluid were compared to those achieved with galactomannan and (1,3)-beta-D-glucan assays. High fungal burden levels were detected by QNRT PCR in both lung tissue and BAL fluid in all infected animals at each time point, and the sensitivity of each assay in BAL fluid was 100% by day 3 and remained so through the remainder of the study. The sensitivity of detection of fungi in whole blood and serum samples was significantly lower, and some samples remained negative by all three assays despite the advanced stage of the infection. From these data, we can conclude that this novel QNRT PCR method was highly sensitive for the detection of A. fumigatus from different types of samples in this model. In addition, BAL fluid samples appeared to be the most suitable for the early diagnosis of invasive pulmonary aspergillosis. When testing serum, the use of a combination of available assays may increase the possibility of early detection of this opportunistic mycosis.
Links
| FR-TI2/254, research and development project |
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