MicroRNA-650 expression is influenced by immunoglobulin gene
rearrangement and affects the biology of chronic lymphocytic
leukemia

J 2012

MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia

MRÁZ, Marek; Dáša DOLEŽALOVÁ; Karla PLEVOVÁ; Kateřina STAŇO KOZUBÍK; Veronika MAYEROVÁ et. al.

Basic information

Original name

MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia

Authors

MRÁZ, Marek (203 Czech Republic, guarantor, belonging to the institution); Dáša DOLEŽALOVÁ (203 Czech Republic, belonging to the institution); Karla PLEVOVÁ (203 Czech Republic, belonging to the institution); Kateřina STAŇO KOZUBÍK (203 Czech Republic, belonging to the institution); Veronika MAYEROVÁ (203 Czech Republic, belonging to the institution); Kateřina ČERNÁ (203 Czech Republic, belonging to the institution); Kateřina MUSILOVÁ (203 Czech Republic, belonging to the institution); Boris TICHÝ (203 Czech Republic, belonging to the institution); Šárka PAVLOVÁ (203 Czech Republic, belonging to the institution); Marek BORSKÝ (203 Czech Republic, belonging to the institution); Jan VERNER (203 Czech Republic, belonging to the institution); Michael DOUBEK (203 Czech Republic, belonging to the institution); Yvona BRYCHTOVÁ (203 Czech Republic, belonging to the institution); Martin TRBUŠEK (203 Czech Republic, belonging to the institution); Aleš HAMPL (203 Czech Republic, belonging to the institution); Jiří MAYER (203 Czech Republic, belonging to the institution) and Šárka POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution)

Edition

Blood, WASHINGTON, Amer Soc Hematology, 2012, 0006-4971

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 9.060

RIV identification code

RIV/00216224:14740/12:00059794

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1182/blood-2011-11-394874

UT WoS

000300949500022

Keywords in English

CLL PATIENTS; SURVIVAL; SUBTYPES

Abstract

V originále

MicroRNAs (miRNAs) play a key role in chronic lymphocytic leukemia as well as in normal B cells. Notably, miRNA gene encoding miR-650 and its homologs overlap with several variable (V) subgenes coding for lambda immunoglobulin (IgL lambda). Recent studies describe the role of miR-650 in solid tumors, but its role in chronic lymphocytic leukemia (CLL) has not yet been studied. Our experiments demonstrate that miR-650 expression is regulated by coupled expression with its host gene for IgL lambda. This coupling provides a unique yet unobserved mechanism for microRNA gene regulation. We determine that higher expression of miR-650 is associated with a favorable CLL prognosis and influences the proliferation capacity of B cells. We also establish that in B cells, miR-650 targets proteins important in cell proliferation and survival: cyclin dependent kinase 1 (CDK1), inhibitor of growth 4 (ING4), and early B-cell factor 3 (EBF3). This study underscores the importance of miR-650 in CLL biology and normal B-cell physiology.

Links

ED1.1.00/02.0068, research and development project

Name: CEITEC - central european institute of technology

MSM0021622430, plan (intention)

Name: Funkční a molekulární charakteristiky nádorových a normálních kmenových buněk - identifikace cílů pro nová terapeutika a terapeutické strategie

Investor: Ministry of Education, Youth and Sports of the CR, Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies

NT11218, research and development project

Name: Funkční a strukturní změny microRNA u lymfoproliferativních malignit a jejich vliv na prognózu onemocnění a predikci léčebné odpovědi

Cite

MRÁZ, Marek; Dáša DOLEŽALOVÁ; Karla PLEVOVÁ; Kateřina STAŇO KOZUBÍK; Veronika MAYEROVÁ; Kateřina ČERNÁ; Kateřina MUSILOVÁ; Boris TICHÝ; Šárka PAVLOVÁ; Marek BORSKÝ; Jan VERNER; Michael DOUBEK; Yvona BRYCHTOVÁ; Martin TRBUŠEK; Aleš HAMPL; Jiří MAYER and Šárka POSPÍŠILOVÁ. MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia. Blood. WASHINGTON: Amer Soc Hematology, 2012, vol. 119, No 9, p. 2110-2113. ISSN 0006-4971. Available from: https://dx.doi.org/10.1182/blood-2011-11-394874.

@article{980555,
   author = {Mráz, Marek and Doležalová, Dáša and Plevová, Karla and Staňo Kozubík, Kateřina and Mayerová, Veronika and Černá, Kateřina and Musilová, Kateřina and Tichý, Boris and Pavlová, Šárka and Borský, Marek and Verner, Jan and Doubek, Michael and Brychtová, Yvona and Trbušek, Martin and Hampl, Aleš and Mayer, Jiří and Pospíšilová, Šárka},
   article_location = {WASHINGTON},
   article_number = {9},
   doi = {http://dx.doi.org/10.1182/blood-2011-11-394874},
   keywords = {CLL PATIENTS; SURVIVAL; SUBTYPES},
   language = {eng},
   issn = {0006-4971},
   journal = {Blood},
   title = {MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia},
   url = {http://bloodjournal.hematologylibrary.org/content/119/9/2110},
   volume = {119},
   year = {2012}
}

TY  - JOUR
ID  - 980555
AU  - Mráz, Marek - Doležalová, Dáša - Plevová, Karla - Staňo Kozubík, Kateřina - Mayerová, Veronika - Černá, Kateřina - Musilová, Kateřina - Tichý, Boris - Pavlová, Šárka - Borský, Marek - Verner, Jan - Doubek, Michael - Brychtová, Yvona - Trbušek, Martin - Hampl, Aleš - Mayer, Jiří - Pospíšilová, Šárka
PY  - 2012
TI  - MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia
JF  - Blood
VL  - 119
IS  - 9
SP  - 2110-2113
EP  - 2110-2113
PB  - Amer Soc Hematology
SN  - 00064971
KW  - CLL PATIENTS
KW  - SURVIVAL
KW  - SUBTYPES
UR  - http://bloodjournal.hematologylibrary.org/content/119/9/2110
L2  - http://bloodjournal.hematologylibrary.org/content/119/9/2110
N2  - MicroRNAs (miRNAs) play a key role in chronic lymphocytic leukemia as well as in normal B cells. Notably, miRNA gene encoding miR-650 and its homologs overlap with several variable (V) subgenes coding for lambda immunoglobulin (IgL lambda). Recent studies describe the role of miR-650 in solid tumors, but its role in chronic lymphocytic leukemia (CLL) has not yet been studied. Our experiments demonstrate that miR-650 expression is regulated by coupled expression with its host gene for IgL lambda. This coupling provides a unique yet unobserved mechanism for microRNA gene regulation. We determine that higher expression of miR-650 is associated with a favorable CLL prognosis and influences the proliferation capacity of B cells. We also establish that in B cells, miR-650 targets proteins important in cell proliferation and survival: cyclin dependent kinase 1 (CDK1), inhibitor of growth 4 (ING4), and early B-cell factor 3 (EBF3). This study underscores the importance of miR-650 in CLL biology and normal B-cell physiology.
ER  -

MRÁZ, Marek; Dáša DOLEŽALOVÁ; Karla PLEVOVÁ; Kateřina STAŇO KOZUBÍK; Veronika MAYEROVÁ; Kateřina ČERNÁ; Kateřina MUSILOVÁ; Boris TICHÝ; Šárka PAVLOVÁ; Marek BORSKÝ; Jan VERNER; Michael DOUBEK; Yvona BRYCHTOVÁ; Martin TRBUŠEK; Aleš HAMPL; Jiří MAYER and Šárka POSPÍŠILOVÁ. MicroRNA-650 expression is influenced by immunoglobulin gene rearrangement and affects the biology of chronic lymphocytic leukemia. \textit{Blood}. WASHINGTON: Amer Soc Hematology, 2012, vol.~119, No~9, p.~2110-2113. ISSN~0006-4971. Available from: https://dx.doi.org/10.1182/blood-2011-11-394874.

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