Electrochemical Study of Doxorubicin Interaction with Different
Sequences of Single Stranded Oligonucleotides, Part I

J 2012

Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I

HYNEK, David, Ludmila KREJČOVÁ, Ondřej ZÍTKA, Vojtěch ADAM, Libuše TRNKOVÁ et. al.

Základní údaje

Originální název

Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I

Autoři

HYNEK, David (203 Česká republika), Ludmila KREJČOVÁ (203 Česká republika), Ondřej ZÍTKA (203 Česká republika), Vojtěch ADAM (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, domácí), Jiří SOCHOR (203 Česká republika), Marie STIBOROVÁ (203 Česká republika), Tomáš ECKSCHLAGER (203 Česká republika), Jaromír HUBÁLEK (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

International Journal of Electrochemical Science, 2012, 1452-3981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Srbsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.729 v roce 2011

Kód RIV

RIV/00216224:14310/12:00060306

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000302723700002

Klíčová slova anglicky

intercalating drug; doxorubicin; hetero-nucleotides; square wave voltammetry; adsorptive transfer stripping technique; DNA

Štítky

AKR, rivok

Změněno: 9. 1. 2013 10:24, Mgr. Sylvie Dohnalíková

Anotace

V originále

Doxorubicin is a known anticancer drug intercalating into dsDNA. The mechanism of interaction of doxorubicin with ssDNA is not clear. In this study, adsorptive transfer stripping technique coupled with square wave voltammetry was used for studying of interaction of doxorubicin with single stranded oligonucleotides (ssODN) in various ratios (1:1, 1:2 and 1:5) and interaction times (0-300 min.). When comparing the results for three ratios of the mixture between drug and ssODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide-doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN, but the signals CA peaks changes during the interaction suggest the changes in the structure of ssODN.

Citovat

HYNEK, David, Ludmila KREJČOVÁ, Ondřej ZÍTKA, Vojtěch ADAM, Libuše TRNKOVÁ, Jiří SOCHOR, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Jaromír HUBÁLEK a René KIZEK. Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I. International Journal of Electrochemical Science. 2012, roč. 7, č. 1, s. 13-33. ISSN 1452-3981.

@article{986083,
   author = {Hynek, David and Krejčová, Ludmila and Zítka, Ondřej and Adam, Vojtěch and Trnková, Libuše and Sochor, Jiří and Stiborová, Marie and Eckschlager, Tomáš and Hubálek, Jaromír and Kizek, René},
   article_number = {1},
   keywords = {intercalating drug; doxorubicin; hetero-nucleotides; square wave voltammetry; adsorptive transfer stripping technique; DNA},
   language = {eng},
   issn = {1452-3981},
   journal = {International Journal of Electrochemical Science},
   title = {Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I},
   volume = {7},
   year = {2012}
}

TY  - JOUR
ID  - 986083
AU  - Hynek, David - Krejčová, Ludmila - Zítka, Ondřej - Adam, Vojtěch - Trnková, Libuše - Sochor, Jiří - Stiborová, Marie - Eckschlager, Tomáš - Hubálek, Jaromír - Kizek, René
PY  - 2012
TI  - Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I
JF  - International Journal of Electrochemical Science
VL  - 7
IS  - 1
SP  - 13-33
EP  - 13-33
SN  - 14523981
KW  - intercalating drug
KW  - doxorubicin
KW  - hetero-nucleotides
KW  - square wave voltammetry
KW  - adsorptive transfer stripping technique
KW  - DNA
N2  - Doxorubicin is a known anticancer drug intercalating into dsDNA. The mechanism of interaction of doxorubicin with ssDNA is not clear. In this study, adsorptive transfer stripping technique coupled with square wave voltammetry was used for studying of interaction of doxorubicin with single stranded oligonucleotides (ssODN) in various ratios (1:1, 1:2 and 1:5) and interaction times (0-300 min.). When comparing the results for three ratios of the mixture between drug and ssODN we found that the interval of peak intensities of oligonucleotide reduced with the increasing ratio of oligonucleotide-doxorubicin, i.e. for ratio 1:1 it is 25-85 %, for ratio 1:2 it is 16-60 % and for ratio 1:5 it is 19-51 %. The interval of relative signal intensities of doxorubicin ranges from 8 to 100 % for ratio 1:1, 22-100 % for ratios 1:2 and 1:5. The results showed the assumption that DOXO peak height increased with increasing concentration of the drug intercalated into ssODN, but the signals CA peaks changes during the interaction suggest the changes in the structure of ssODN.
ER  -

HYNEK, David, Ludmila KREJČOVÁ, Ondřej ZÍTKA, Vojtěch ADAM, Libuše TRNKOVÁ, Jiří SOCHOR, Marie STIBOROVÁ, Tomáš ECKSCHLAGER, Jaromír HUBÁLEK a René KIZEK. Electrochemical Study of Doxorubicin Interaction with Different Sequences of Single Stranded Oligonucleotides, Part I. \textit{International Journal of Electrochemical Science}. 2012, roč.~7, č.~1, s.~13-33. ISSN~1452-3981.

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