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@article{987839, author = {Kohoutek, Jiří and Blažek, Dalibor and Peterlin, B Matija}, article_location = {WASHINGTON}, article_number = {46}, doi = {http://dx.doi.org/10.1073/pnas.0603079103}, keywords = {RNA-POLYMERASE-II; MELANOMA CELL-LINES; P-TEFB; 7SK SNRNA; EXPRESSION; CIITA; COMPLEX; GENES; DIFFERENTIATION; ACTIVATION}, language = {eng}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, title = {Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters}, url = {http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1859933/}, volume = {103}, year = {2006} }
TY - JOUR ID - 987839 AU - Kohoutek, Jiří - Blažek, Dalibor - Peterlin, B Matija PY - 2006 TI - Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters JF - Proceedings of the National Academy of Sciences of the United States of America VL - 103 IS - 46 SP - 17349-17354 EP - 17349-17354 PB - NATL ACAD SCIENCES SN - 00278424 KW - RNA-POLYMERASE-II KW - MELANOMA CELL-LINES KW - P-TEFB KW - 7SK SNRNA KW - EXPRESSION KW - CIITA KW - COMPLEX KW - GENES KW - DIFFERENTIATION KW - ACTIVATION UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1859933/ N2 - The class II transactivator (CIITA) is the master integrator of expression of MHC class II genes. It interacts with variety of basal transcription factors to initiate and elongate transcription of these genes. Among others, it recruits positive transcription elongation factor b (P-TEFb) to MHC class II promoters. In cells, P-TEFb is found in small active or large inactive complexes. The large complex is composed of P-TEFb, 7SK small nuclear RNA, and hexamethylene bisacetamide-inducible protein 1 (Hexim1). The present study identifies Hexim1 as a potent inhibitor of CIITA-mediated transcription. Not only the exogenously expressed but also IFN-gamma-induced CIITA was inhibited by Hexim1. This inhibition did riot result from an association between Hexim1 and CIITA but depended on the intact Cyclin T1-binding domain in Hexim1. importantly, Hexim1 sequestered P-TEFb from CIITA, as documented by binding competition and ChIP assays. Conversely, the depletion of Hexim1 from cells by siRNA increased CIITA-mediated transcription. Thus, modulating ratios between active and inactive P-TEFb complexes is an additional mechanism of regulating transcriptional activators such as CIITA. ER -
KOHOUTEK, Jiří, Dalibor BLAŽEK a B Matija PETERLIN. Hexim1 sequesters positive transcription elongation factor b from the class II transactivator on MHC class II promoters. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: NATL ACAD SCIENCES, 2006, roč.~103, č.~46, s.~17349-17354. ISSN~0027-8424. Dostupné z: https://dx.doi.org/10.1073/pnas.0603079103.
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