Knockdown of apoptosis-inducing factor disrupts function of
respiratory complex I

J 2012

Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I

VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO et. al.

Základní údaje

Originální název

Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I

Název česky

Snížení množství apoptózu vyvolávajícího faktoru narušuje funkci dýchacího komplexu I

Autoři

VAŘECHA, Miroslav (203 Česká republika, garant, domácí), Daniela PÁCLOVÁ (203 Česká republika, domácí), Jiřina PROCHÁZKOVÁ (203 Česká republika, domácí), Pavel MATULA (203 Česká republika, domácí), Dušan CMARKO (203 Česká republika) a Michal KOZUBEK (203 Česká republika, domácí)

Vydání

BIOCELL, Argentina, National Council of Scientific and Techn, 2012, 0327-9545

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Argentina

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.575

Kód RIV

RIV/00216224:14330/12:00057522

Organizační jednotka

Fakulta informatiky

UT WoS

000328358900004

Klíčová slova česky

superoxid;fluorescenční mikroskopie;obrazová analýza;mitochondrie;telomeráza

Klíčová slova anglicky

superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 2. 2013 09:59, Mgr. Miroslav Vařecha, Ph.D.

Anotace

V originále

Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide production in comparison to control cells, while a non-tumoral cell line and tumoral telomerase-negative cell lines with alternative lengthening of telomeres (ALT) show a decrease in superoxide production. According to these results, we can conclude that AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria. The distinct effect of AIF depletion in various cell lines could result from recently discovered activity of telomerase in mitochondria of TP cancer cells, but this hypothesis needs further investigation.

Návaznosti

GBP302/12/G157, projekt VaV

Název: Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Investor: Grantová agentura ČR, Dynamika a organizace chromosomů během buněčného cyklu a při diferenciaci v normě a patologii

Přiložené soubory

04_Varecha.pdf

Požádat o autorskou verzi souboru

Citovat

VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO a Michal KOZUBEK. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. BIOCELL. Argentina: National Council of Scientific and Techn, 2012, roč. 36, č. 3, s. 121-126. ISSN 0327-9545.

@article{989665,
   author = {Vařecha, Miroslav and Páclová, Daniela and Procházková, Jiřina and Matula, Pavel and Cmarko, Dušan and Kozubek, Michal},
   article_location = {Argentina},
   article_number = {3},
   keywords = {superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase},
   language = {eng},
   issn = {0327-9545},
   journal = {BIOCELL},
   note = {Accepted.},
   title = {Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I},
   url = {http://www.scielo.org.ar/scielo.php?script=sci_issues&pid=0327-9545&lng=en&nrm=iso},
   volume = {36},
   year = {2012}
}

TY  - JOUR
ID  - 989665
AU  - Vařecha, Miroslav - Páclová, Daniela - Procházková, Jiřina - Matula, Pavel - Cmarko, Dušan - Kozubek, Michal
PY  - 2012
TI  - Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I
JF  - BIOCELL
VL  - 36
IS  - 3
SP  - 121-126
EP  - 121-126
PB  - National Council of Scientific and Techn
SN  - 03279545
N1  - Accepted.
KW  - superoxide;fluorescence microscopy;image analysis;mitochondria;telomerase
UR  - http://www.scielo.org.ar/scielo.php?script=sci_issues&pid=0327-9545&lng=en&nrm=iso
N2  - Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide production in comparison to control cells, while a non-tumoral cell line and tumoral telomerase-negative cell lines with alternative lengthening of telomeres (ALT) show a decrease in superoxide production. According to these results, we can conclude that AIF knockdown disrupts function of complex I and therefore increases the superoxide production in mitochondria. The distinct effect of AIF depletion in various cell lines could result from recently discovered activity of telomerase in mitochondria of TP cancer cells, but this hypothesis needs further investigation.
ER  -

VAŘECHA, Miroslav, Daniela PÁCLOVÁ, Jiřina PROCHÁZKOVÁ, Pavel MATULA, Dušan CMARKO a Michal KOZUBEK. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. \textit{BIOCELL}. Argentina: National Council of Scientific and Techn, 2012, roč.~36, č.~3, s.~121-126. ISSN~0327-9545.

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