Screening a cDNA Library for Protein-Protein Interactions
Directly in Planta

J 2012

Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH et. al.

Basic information

Original name

Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

Authors

LEE, Lan-Ying (158 Taiwan), Fu-Hui WU (158 Taiwan), Chen-Tran HSU (158 Taiwan), Shu-Chen SHEN (158 Taiwan), Hsuan-Yu YEH (158 Taiwan), De-Chih LIAO (158 Taiwan), Mei-Jane FANG (158 Taiwan), Nien-Tze LIU (158 Taiwan), Yu-Chen YEN (158 Taiwan), Ladislav DOKLÁDAL (203 Czech Republic, belonging to the institution), Eva SÝKOROVÁ (203 Czech Republic, guarantor, belonging to the institution), Stanton B. GELVIN (840 United States of America) and Choun-Sea LIN (158 Taiwan)

Edition

Plant Cell, UNITED STATES, AMER SOC PLANT BIOLOGISTS, 2012, 1040-4651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.251

RIV identification code

RIV/00216224:14740/12:00057108

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1105/tpc.112.097998

UT WoS

000306105400008

Keywords in English

BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION

Abstract

V originále

Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.

Links

IAA500040801, research and development project

Name: Telomery a telomerázy: posun od molekulárně-biologického ke strukturně-biologickému přístupu.

Investor: Academy of Sciences of the Czech Republic, Telomeres and telomerase: transition from molecular to structural biology approach

Citovat

LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH, De-Chih LIAO, Mei-Jane FANG, Nien-Tze LIU, Yu-Chen YEN, Ladislav DOKLÁDAL, Eva SÝKOROVÁ, Stanton B. GELVIN and Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. Plant Cell. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, vol. 24, No 5, p. 1746-1759. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.097998.

@article{990393,
   author = {Lee, LanandYing and Wu, FuandHui and Hsu, ChenandTran and Shen, ShuandChen and Yeh, HsuanandYu and Liao, DeandChih and Fang, MeiandJane and Liu, NienandTze and Yen, YuandChen and Dokládal, Ladislav and Sýkorová, Eva and Gelvin, Stanton B. and Lin, ChounandSea},
   article_location = {UNITED STATES},
   article_number = {5},
   doi = {http://dx.doi.org/10.1105/tpc.112.097998},
   keywords = {BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Screening a cDNA Library for Protein-Protein Interactions Directly in Planta},
   url = {http://www.plantcell.org/content/24/5/1746.abstract},
   volume = {24},
   year = {2012}
}

TY  - JOUR
ID  - 990393
AU  - Lee, Lan-Ying - Wu, Fu-Hui - Hsu, Chen-Tran - Shen, Shu-Chen - Yeh, Hsuan-Yu - Liao, De-Chih - Fang, Mei-Jane - Liu, Nien-Tze - Yen, Yu-Chen - Dokládal, Ladislav - Sýkorová, Eva - Gelvin, Stanton B. - Lin, Choun-Sea
PY  - 2012
TI  - Screening a cDNA Library for Protein-Protein Interactions Directly in Planta
JF  - Plant Cell
VL  - 24
IS  - 5
SP  - 1746-1759
EP  - 1746-1759
PB  - AMER SOC PLANT BIOLOGISTS
SN  - 10404651
KW  - BIMOLECULAR FLUORESCENCE COMPLEMENTATION
KW  - STRAND TELOMERIC DNA
KW  - BINDING-PROTEIN
KW  - NUCLEAR IMPORT
KW  - IN-PLANTA
KW  - T-DNA
KW  - VIRD2 PROTEIN
KW  - CELLS
KW  - TRANSFORMATION
KW  - VISUALIZATION
UR  - http://www.plantcell.org/content/24/5/1746.abstract
L2  - http://www.plantcell.org/content/24/5/1746.abstract
N2  - Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.
ER  -

LEE, Lan-Ying, Fu-Hui WU, Chen-Tran HSU, Shu-Chen SHEN, Hsuan-Yu YEH, De-Chih LIAO, Mei-Jane FANG, Nien-Tze LIU, Yu-Chen YEN, Ladislav DOKLÁDAL, Eva SÝKOROVÁ, Stanton B. GELVIN and Choun-Sea LIN. Screening a cDNA Library for Protein-Protein Interactions Directly in Planta. \textit{Plant Cell}. UNITED STATES: AMER SOC PLANT BIOLOGISTS, 2012, vol.~24, No~5, p.~1746-1759. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.112.097998.

Detailed Information on Publication Record