J 2012

Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus

DOSPIVOVÁ, Dana, David HYNEK, Pavel KOPEL, Andrea BEZDĚKOVÁ, Jiří SOCHOR et. al.

Základní údaje

Originální název

Electrochemical Behaviour of Apoferritin Encapsulating of Silver(I) Ions and Its Application for Treatment of Staphylococcus aureus

Autoři

DOSPIVOVÁ, Dana (203 Česká republika), David HYNEK (203 Česká republika), Pavel KOPEL (203 Česká republika), Andrea BEZDĚKOVÁ (203 Česká republika), Jiří SOCHOR (203 Česká republika), Soňa KŘÍŽKOVÁ (203 Česká republika), Vojtěch ADAM (203 Česká republika), Libuše TRNKOVÁ (203 Česká republika, domácí), Jaromír HUBÁLEK (203 Česká republika), Petr BABULA (203 Česká republika), Ivo PROVAZNÍK (203 Česká republika), Radimír VRBA (203 Česká republika) a René KIZEK (203 Česká republika, garant)

Vydání

International Journal of Electrochemical Science, 2012, 1452-3981

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Srbsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.729 v roce 2011

Kód RIV

RIV/00216224:14310/12:00060702

Organizační jednotka

Přírodovědecká fakulta

UT WoS

000306399700051

Klíčová slova anglicky

electrochemical detection; silver; nanomaterial; nanomedicine

Štítky

Změněno: 9. 1. 2013 10:18, Mgr. Sylvie Dohnalíková

Anotace

V originále

Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for several difficult-to-treat infections in humans. Therefore, it is not surprising that other ways how to treat these bacteria are looked for. Silver(I) ions and silver nanoparticles exhibited the highest antimicrobial activity against MRSA but their transporting to the place of needs and in situ determination is an issue. The aim of this work was electrochemical determination of silver(I) ions using four types of modified carbon paste electrodes (CPEs) with different content of carbon nanoparticles. CPE made from expanded carbon was the most sensitive one. Therefore, we optimized the experimental conditions as time of accumulation 60 s, deposition potential 0.5 V and 0.2 M acetate buffer, pH = 5.0 to obtain detection limit (3 S/N) of 5 nM for silver(I) ions. Further, we studied the encapsulation of silver(I) ions into apoferritin as a possible way for transportation of these ions. Primarily we optimized the encapsulation conditions to prepare the most stable complex, which was subsequently utilized for treatment of S. aureus. Based on the results obtained it can be concluded that silver(I) ions remain enclosed in the apoferritin structure until decomposition of apoferritin by bacterial enzymatic apparatus occurs.