Importance of lipid binding for elicitin biological activity

a 2010

Importance of lipid binding for elicitin biological activity

KLEMPOVÁ, Jitka, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN

Základní údaje

Originální název

Importance of lipid binding for elicitin biological activity

Autoři

KLEMPOVÁ, Jitka, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN

Vydání

XIV. Setkání biochemiků a molekulárních biologů. Brno. 2010

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

ISBN

978-80-210-5164-5

Změněno: 3. 9. 2012 08:51, Mgr. Jitka Klempová

Anotace

V originále

Within the process of evolution plants were in contact with wide range of potential pathogens, including viruses, bacteria, fungi, oomycetes, nematodes or insects. So, consequently plants have developed various strategies to defence themselves from potential attack. Plant pathogen attacks are perceived through pathogen-issued compounds or plant-derived molecules that elicit defence reactions. We have applied as a model interaction of basic elicitin cryptogein, produced by Phytophthora cryptogea, with plants Nicotiana tabacum. Cryptogein is a small 10kDa holoprotein inducing on tobacco plants cascade of defence responses including protein phosphorylations, changes in ion fluxes across the plasma membrane, production of active oxygen species, nitric oxide, phytoalexins, and reinforcement of cell wall or expression of pathogenesis related proteins. Usually all these signalling events could contribute to development of systemic acquired resistance to a wide spectrum of pathogen in non-infected parts of plant. However, the precise biological role of elicitins is still not known their ability to bind sterols and fatty acids, has been described. The cryptogein-sterol complex was suggested to be necessary for triggering of early events such as active oxygen species production or pH changes. On the other hand the ability to stimulate necrosis and expression of defence proteins seems to be not dependent on sterol binding. To verify this theory a series of cryptogein mutants M35W/M59W, L19R and L15W/L36F with altered abilities to bind sterols and fatty acids were prepared. A clear relationship between the caspidiol production, sterol binding and AOS production was demonstrated. However, in the case of expression of genes for pathogenesis related (PR) proteins of classes 1, 3 and 5 different patterns were observed in relation to sterol binding properties. Moreover, only in PR1a protein a clear relation between the SA accumulation and gene expression was found.

Citovat

KLEMPOVÁ, Jitka, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN. Importance of lipid binding for elicitin biological activity. In XIV. Setkání biochemiků a molekulárních biologů. Brno. 2010. ISBN 978-80-210-5164-5.

@proceedings{990736,
   author = {Klempová, Jitka and Kašparovský, Tomáš and Šerý, Omar and Lochman, Jan},
   booktitle = {XIV. Setkání biochemiků a molekulárních biologů. Brno.},
   language = {eng},
   isbn = {978-80-210-5164-5},
   title = {Importance of lipid binding for elicitin biological activity},
   year = {2010}
}

TY  - CONF
ID  - 990736
AU  - Klempová, Jitka - Kašparovský, Tomáš - Šerý, Omar - Lochman, Jan
PY  - 2010
TI  - Importance of lipid binding for elicitin biological activity
SN  - 9788021051645
N2  - Within the process of evolution plants were in contact with wide range of potential pathogens, including viruses, bacteria, fungi, oomycetes, nematodes or insects. So, consequently plants have developed various strategies to defence themselves from potential attack. Plant pathogen attacks are perceived through pathogen-issued compounds or plant-derived molecules that elicit defence reactions. We have applied as a model interaction of basic elicitin cryptogein, produced by Phytophthora cryptogea, with plants Nicotiana tabacum. Cryptogein is a small 10kDa holoprotein inducing on tobacco plants cascade of defence responses including protein phosphorylations, changes in ion fluxes across the plasma membrane, production of active oxygen species, nitric oxide, phytoalexins, and reinforcement of cell wall or expression of pathogenesis related proteins. Usually all these signalling events could contribute to development of systemic acquired resistance to a wide spectrum of pathogen in non-infected parts of plant. However, the precise biological role of elicitins is still not known their ability to bind sterols and fatty acids, has been described. The cryptogein-sterol complex was suggested to be necessary for triggering of early events such as active oxygen species production or pH changes. On the other hand the ability to stimulate necrosis and expression of defence proteins seems to be not dependent on sterol binding. To verify this theory a series of cryptogein mutants M35W/M59W, L19R and L15W/L36F with altered abilities to bind sterols and fatty acids were prepared. A clear relationship between the caspidiol production, sterol binding and AOS production was demonstrated. However, in the case of expression of genes for pathogenesis related (PR) proteins of classes 1, 3 and 5 different patterns were observed in relation to sterol binding properties. Moreover, only in PR1a protein a clear relation between the SA accumulation and gene expression was found.
ER  -

KLEMPOVÁ, Jitka, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN. Importance of lipid binding for elicitin biological activity. In \textit{XIV. Setkání biochemiků a molekulárních biologů. Brno.}. 2010. ISBN~978-80-210-5164-5.

Podrobný výpis o publikaci