BLLM0321p Medical Microbiology I - lecture

Faculty of Medicine
autumn 2022
Extent and Intensity
2/0/0. 0 credit(s). Type of Completion: -.
Teacher(s)
MUDr. Lenka Černohorská, Ph.D. (lecturer)
Mgr. Monika Dvořáková Heroldová, Ph.D. (lecturer)
prof. MUDr. Filip Růžička, Ph.D. (lecturer)
doc. MUDr. Vladana Woznicová, Ph.D. (lecturer)
MUDr. Ondřej Zahradníček (lecturer)
Michaela Gregorovičová (assistant)
Iva Holešovská (assistant)
Guaranteed by
prof. MUDr. Filip Růžička, Ph.D.
Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: MUDr. Ondřej Zahradníček
Supplier department: Department of Microbiology – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable
Fri 16. 9. 9:30–11:10 MiÚ N04308, Fri 23. 9. 9:30–11:10 MiÚ N04308, Fri 30. 9. 9:30–11:10 MiÚ N04308, Fri 7. 10. 9:30–11:10 MiÚ N04308, Fri 14. 10. 9:30–11:10 MiÚ N04308, Fri 21. 10. 9:30–11:10 MiÚ N04308, Fri 4. 11. 9:30–11:10 MiÚ N04308, Fri 11. 11. 9:30–11:10 MiÚ N04308, Fri 18. 11. 9:30–11:10 MiÚ N04308, Fri 25. 11. 9:30–11:10 MiÚ N04308, Fri 2. 12. 9:30–11:10 MiÚ N04308, Fri 9. 12. 9:30–11:10 MiÚ N04308, Fri 16. 12. 9:30–11:10 MiÚ N04308, Fri 23. 12. 9:30–11:10 MiÚ N04308
Prerequisites
Students are supposed to know basics of biology of living organisms.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to get the students acquainted with the practical implementation of individual methods of medical microbiology and the safe acquisition of these methods.
Learning outcomes
At the end of this course, students should be able to understand principles of following diagnostic methods in medical microbiology:
microscopy (wet mount, Gram staining, other staining methods)
culture (culture media, theire use, differences between them)
identification of bacteria and yeasts (various methods of biochemical identification, other identification methods)
antibiotic susceptibility/resistance testing methods
animal experiment (only basically)
nucleic acid detection in microbiology (only basically)
methods detecting antigens or antibodies, theire use for antibody detection/antigen detection in specimen/antigen analysis of a strain; titers, titer dynamics, detection of IgM/IgA/IgG; precipitation, agglutination, agglutination on carriers, complementfixing test, neutralisation reaction, immunofluorescence, radioimmunoassay, ELISA, Western blotting

They will also understand basics of:
microbial morpohology and physiology, basics of microbial genetics
pathogenicity and virulence, pathogenesis and course of an infection
antimicrobial immunity, active and passive immunisation
tenacity of microorganisms, basic principles and practical use of decontamination methods
therapy using antimicrobial drugs, possible principles of effect and practical use
epidemiology of infectious diseases, individual routes of infections, terms "source of infection", "vector", "vehiculum" and their importance
Syllabus
  • 1. Introduction, medically important microbes, targets and methodology of clinical bacteriology.
  • 2. Morphology and composition of bacteria. Principle of staining techniques globally, Gram staining, special microscopical and staining techniques
  • 3. Metabolism and multiplication of bacteria. Culture techniques (types of media)
  • 4. Methods of bacterial identification, basic principles of biochemical tests
  • 5. Antibiotics I
  • 6. Antibiotics II
  • 7. Molecular methods in microbiology. Animal experiment in microbiology (including ethical frame)
  • 8. Pathogenicity and virulence, course and forms of infection
  • 9. Basics of immunology in microbiology
  • 10. Introduction to serology, agglutination and precipitation, agglutination on carriers
  • 11. Complementfixing test
  • 12. Neutralisation reactions
  • 13. Reactions with labelled components, Western blotting
  • 14. Microbes and outer environment - basics of disinfection and sterilisation
  • 15. Routes of transmission of infectious agents
  • (16. - 30. see BLLM0322p)
Literature
    recommended literature
  • VOTAVA, Miroslav. Lékařská mikrobiologie : vyšetřovací metody. Brno: Neptun, 2010, 495 s. ISBN 9788086850047. info
  • Prezentace z přednášek
Teaching methods
lecture
self-study with use of lecture slide-shows
Assessment methods
BLLM0321p is not finished by credit or examination. The final examination follows after the second block of lectures (BLLM0322p). It is an examination composed of a practical task and answering a triplet of questions (there are 120 questions totally).
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2017, autumn 2018, autumn 2019, autumn 2020, autumn 2021, autumn 2023, autumn 2024.
  • Enrolment Statistics (autumn 2022, recent)
  • Permalink: https://is.muni.cz/course/med/autumn2022/BLLM0321p