EMZM0511p Modern imaging and analytical methods - lecture

Faculty of Medicine
autumn 2024
Extent and Intensity
2/0/0. 4 credit(s). Type of Completion: zk (examination).
Teacher(s)
prof. Mgr. Jan Preisler, Ph.D. (lecturer)
prof. RNDr. Viktor Kanický, DrSc. (lecturer)
doc. Mgr. Tomáš Vaculovič, Ph.D. (lecturer)
prof. RNDr. Přemysl Lubal, Ph.D. (lecturer)
doc. Mgr. Petr Táborský, Ph.D. (lecturer)
doc. Mgr. Karel Novotný, Ph.D. (lecturer)
Mgr. Aleš Hrdlička, Ph.D. (lecturer)
doc. RNDr. Jiří Urban, Ph.D. (lecturer)
Mgr. Anna Týčová, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Jan Preisler, Ph.D.
Department of Chemistry – Chemistry Section – Faculty of Science
Supplier department: Department of Chemistry – Chemistry Section – Faculty of Science
Timetable
Tue 13:00–14:40 B11/327
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to provide the student with sufficiently detailed information about the rapidly developing methods of analyzing the chemical composition of living structures, which are increasingly being applied in clinical medicine and are expected to be used in the field of human reproduction as well.
Learning outcomes
After completing the course, the student will be able to: - Understand the chemical and physical principles of the relevant analytical methods, - Assess the basic parameters of the data obtained by these methods, - Navigate relevant instrumentation.
Syllabus
  • The lecture series will cover the following topics: **Introduction (Jan Preisler)** 1. **Introduction to Analytical Chemistry (Tomáš Vaculovič):** Definition and role of analytical chemistry. General analysis procedures. Errors and statistical data processing. 2. **Theoretical Foundations of Analytical Chemistry (Viktor Kanický):** Chemical analysis of solutions and solid phases. Solutions and dissolution. Analytical reactions. Protonic, complex-forming, precipitative, and redox reactions. Qualitative and quantitative analysis. Basics of gravimetric and volumetric analysis. 3. **Molecular Absorption Spectroscopy in UV/Vis/IR Regions. Raman Spectroscopy (Přemysl Lubal):** Brief theoretical foundations – basic concepts, description of instrumentation, practical examples of use in clinical analysis. 4. **NMR Spectrometry – Magnetic Resonance Imaging (MRI) (Přemysl Lubal):** Brief theoretical foundations – basic concepts of both methods, description of instrumentation, practical examples of use in clinical analysis, MRI imaging. 5. **Fluorescence Spectrometry and Microscopy (Petr Táborský):** Luminescence, Jablonski diagram. Emission and excitation spectra. Fluorescence properties of molecules. Fluorescent labels and probes. Spectrofluorometer and fluorescence microscope. Time-resolved fluorescence and FLIM. 6. **Atomic Spectrometry (Karel Novotný):** Absorption and emission of electromagnetic radiation by free atoms and ions. Principles of AAS and methods of atomization. Absorption lines. Excitation methods in OES and emission spectra. Qualitative and quantitative analysis. Laser-induced breakdown spectroscopy (LIBS). Application of atomic spectrometry in imaging techniques. 7. **Mass Spectrometry (Jan Preisler):** Introduction to mass spectrometry. Ionization techniques. Mass analyzers. Ion mobility. Coupling with separation techniques. Selected applications. Imaging mass spectrometry. 8. **Imaging Methods Using Radioisotopes. Analytical and Imaging Techniques Based on X-ray Radiation (Přemysl Lubal):** Basic concepts in radiochemistry, fundamentals of instrumentation, SPECT/PET imaging techniques and their applications in medicine. Absorption and diffraction of X-ray radiation and their use in chemical and pharmaceutical analysis. Basics of instrumentation. Imaging techniques (simple X-ray, CT). 9. **Electron Microscopy, SPM: STM, AFM (Aleš Hrdlička):** Principles and applications of scanning electron microscopy, electron microprobes with excitation and energy-dispersive X-ray detection, Auger-Meitner electrons, elemental mapping of samples. Basics of scanning probe microscopy, tunneling microscopy, and atomic force microscopy. 10. **Electroanalytical Methods (Libuše Trnková):** Electrochemical, electrode, and membrane potentials. Advanced electroanalytical methods and their applications in biopolymer research. Electrochemical sensors, biosensors, and nanosensors. 11. **Chromatography (Jiří Urban):** Basics of the separation process, retention mechanisms and their description. Types of stationary phases. Retention characteristics and their evaluation. Isocratic and gradient elution. Supercritical fluid chromatography. 12. **Electromigration Methods (Anna Týčová):** Principles of substance movement in an electric field. General overview of electromigration methods. Capillary zone electrophoresis. Isoelectric focusing. Gel electrophoresis. Isotachophoresis. Electrochromatography. Microfluidic setups for electromigration methods.
Literature
    required literature
  • Výchozím materiálem ke studiu je prezentace, která je studentům k dispozici i v elektronické podobě.
Teaching methods
lectures held by specialists
Assessment methods
written exam
Language of instruction
Czech
Further comments (probably available only in Czech)
Study Materials
Information on the extent and intensity of the course: 30.
The course is also listed under the following terms autumn 2022, autumn 2023.
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/med/autumn2024/EMZM0511p