BLMT0211p Toxicological Techniques - lecture

Faculty of Medicine
Spring 2018
Extent and Intensity
2/0/0. 3 credit(s). Type of Completion: zk (examination).
Teacher(s)
Mgr. Andrea Brzobohatá, Ph.D. (lecturer)
Michaela Gregorovičová (assistant)
Guaranteed by
Mgr. Andrea Brzobohatá, Ph.D.
Department of Forensic Medicine – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Contact Person: Mgr. Andrea Brzobohatá, Ph.D.
Supplier department: Department of Forensic Medicine – Institutions shared with St. Anne's Faculty Hospital – Faculty of Medicine
Timetable
Mon 10:00–11:40 ÚSL N03088
Prerequisites (in Czech)
BLLC0111s Med.Chem.-seminar && ZC011 Handling chemical substances
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
The aim of this subject is to acquaint students with principles of toxicological examining methods used in clinical, industrial, hygienic and pharmacological toxicology. They are physiochemical methods used for proof and determination of toxic substances, medicaments, eventually their metabolites in stomach and intestine content, in bodily secretions and excretions, in all bodily fluids and organs with the aim to assess the cuase of poisoning.
Learning outcomes
acquiring knowledge of clinical toxicology, possibilities of examining biological material, drug detection and inclusion in patient diagnosis
Syllabus
  • Toxicity of the substance; Principles of the Good laboratory practice; Principles of the proper sampling, transporting and preservation of biological symplex; Metabolic pathways of substances, pharmacokinetics and pharmacodynamics ; Biological material treatment for toxicological analysis (types of the biological material, sensory examination, deproteinisation, isolation – elimination of balast substances, transfer to the organic phase, preconcentration, diffusion methods, mineralisation, vapour distillation, atc.); Volatile substances, determination of ethanol, head-space – methods, metabolites; Inorganic substances, acids, bases, metalic substances, cyanides, carbon monooxide; Extractable substances, medicaments, drugs of abuse, agrochemicals, evidence; Principles of the isolation (separation) methods (extraction liquid-liquid, solid-phase extraction, microextraction, effect of pH, hydrolysis); Methods of isolation of unknown noxa (toxic, narcotic and psychotropic substances in biological material); Principles of immuno-assay tests for proof of unknown toxic substance (Systematic toxicological analysis, immunochemistry, TLC, GC/MS, HPLC); Principles of substance identification, GC/MS, derivatization, target analysis; Principles of methods for determination of concentrations of medicaments, volatile toxic substances, narcotic and psychotropic substances (GC, HPLC, CE, specific detectors, standards, atc.); Biological and exposure tests (testing of substance toxicity, testing on animals, determination of noxa metabolites in urine, resp. in blood related to exposition to some substances in workplace; Profession testing, cut-off – values; Alternative materials (hair, saliva, sweat, meconium); Biological proof of toxic substance; Interferences in toxicological practice (immunochemistry, cross-reactivity, physiological levels of endogenous substances: hippuric acid, urea, foodstuff additives, phthalates, disinfection artefacts, poppy diet); Internal and external quality control in toxicology (validation of methods, precision, accuracy, reproducibility, cut-off – values, internal standards, comparative blank samples, skill testing – unknown sample, proof by two independent methods at least); Laboratory calculations in toxikology; Statistical data treatment in toxikology; Fundamentals of method establishment in toxicology ;
Literature
Teaching methods
lecture
Assessment methods
examination
Language of instruction
Czech
Listed among pre-requisites of other courses
The course is also listed under the following terms Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2019, spring 2020, spring 2021, spring 2022, spring 2023, spring 2024, spring 2025.
  • Enrolment Statistics (Spring 2018, recent)
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