F1BC1_16 Biochemistry

Faculty of Pharmacy
Autumn 2022
Extent and Intensity
3/1/0. 6 credit(s). Type of Completion: zk (examination).
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Ing. Branislav Ruttkay-Nedecký, Dr. (lecturer)
PharmDr. Zuzana Soldánová (lecturer)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
Ing. Branislav Ruttkay-Nedecký, Dr. (seminar tutor)
PharmDr. Zuzana Soldánová (seminar tutor)
Mgr. Filip Křikava (seminar tutor)
Mgr. Tereza Mikešová (seminar tutor)
Mgr. Denis Šubert (seminar tutor)
Guaranteed by
Mgr. Marie Brázdová, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Supplier department: Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Thu 13:00–15:30 45-135
  • Timetable of Seminar Groups:
F1BC1_16/01: Tue 13. 9. 7:30–10:25 45-229, Tue 4. 10. 7:30–10:25 45-229, Tue 25. 10. 7:30–10:25 45-229, Tue 15. 11. 7:30–10:25 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
F1BC1_16/02: Tue 20. 9. 7:30–10:25 45-229, Tue 11. 10. 7:30–10:25 45-229, Tue 1. 11. 7:30–10:25 45-229, Tue 22. 11. 7:30–10:25 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
F1BC1_16/03: Tue 27. 9. 7:30–10:25 45-229, Tue 18. 10. 7:30–10:25 45-229, Tue 8. 11. 7:30–10:25 45-229, Tue 29. 11. 7:30–10:25 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
F1BC1_16/04: Tue 13. 9. 10:40–13:35 45-229, Tue 4. 10. 10:40–13:35 45-229, Tue 25. 10. 10:40–13:35 45-229, Tue 15. 11. 10:40–13:35 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
F1BC1_16/05: Tue 20. 9. 10:40–13:35 45-229, Tue 11. 10. 10:40–13:35 45-229, Tue 1. 11. 10:40–13:35 45-229, Tue 22. 11. 10:40–13:35 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
F1BC1_16/06: Tue 27. 9. 10:40–13:35 45-229, Tue 18. 10. 10:40–13:35 45-229, Tue 8. 11. 10:40–13:35 45-229, Tue 29. 11. 10:40–13:35 45-229, M. Brázdová, T. Mikešová, B. Ruttkay-Nedecký, Z. Soldánová, D. Šubert
Prerequisites
FAKULTA ( FaF ) || OBOR ( MUSFaF )
The knowledge of subject in range of lectures
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Biochemistry is the study of the chemical processes (changes) which condition and accompany life in its most varied forms and manifestations, and attemps to explain its essence on a molecular level. Medical biochemistry is biochemistry in use, applied quite specifically in veterinary medicine (for example, the peculiarities of the metabolism of nutrients in invidual species of livestock). Further, in teaching of biochemistry, it is necessary to concentrate on detailed interpretation of selected chapters, that are most significant for the formation of the European profile of a graduate of veterinary university. Practical (laboratory) work where verbal information is confirmed by practical experience is also an integral component of the biochemistry teaching.

Learning outcomes
Basic knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology, Pharmacogenomics, Structure and evolution of genomes.
Syllabus
  • Syllabus Biochemistry
    1) Biochemistry: an introduction
    1.1) Scope of biochemistry: central principles, cellular and chemical foundations (functional groups, reactions), physical foundations
    1.2) Living cells (structure of eukaryotic cell)
    1.3) Water: the medium of life (interactions, pH, pKa, buffers, blood, liver and urine buffering systems, osmosis, dialysis)
    1.4) Energy (free energy, ATP)
    2) Structure and function of proteins
    2.1) Amino acids (structure, pKa, pI, properties, covalent modifications)
    2.2) Peptides (peptide bound, disulfide bridge, glutathione, insulin, antibiotics, neurotransmiters)
    2.3) Protein structure (secondary, tertiary, conformation, structure and function)
    2.4) Structure and function of hemoglobin and myoglobin
    3) Enzymes
    3.1) Properties of Enzymes (cofactors, coenzymes, vitamins)
    3.2) Classification of Enzymes (examples of each class)
    3.3) Catalysis (enzyme reaction, active site, specificity) and Enzyme regulation
    3.4) Kinetics of enzyme reactions
    4) Membrane transport
    4.1) Biomembranes - composition and function
    4.2) Membrane transports
    5) Carbohydrates and metabolism of carbohydrate
    5.1) Monosaccharides, Disaccharides, Oligosaccharides
    5.2) Polysaccharides (structure and function)
    5.3) Metabolism, homeostasis, catabolism, anabolism, nutrient pool, energy metabolism
    5.4) Glycolysis and Glyconeogenesis
    5.5) Glycogen metabolism
    6) Metabolism of Proteins and Amino Acid Nitrogen
    6.1) Overview of metabolism of proteins,
    6.2) Elimination of alfa-amino nitrogen
    6.3) Detoxification of Ammonia
    6.4) Catabolism of Amino Acids (Ala, Arg, Ser, Gly, Thr, Asp, Glu, His, Phe, Tyr, Cys)
    7) Aerobic metabolism
    6.1) Tricarboxy Acid Cycle (Citric Acid Cycle (overview, fuels, mitochondrion, enzymes and coenzymes, 8 steps of CC, regulation of pyruvate dehydrogenase, control of CC, biosynthesis aspects of CC)
    6.2) Respiratory chain (redox potential, sequence of electron carriers)
    6.3) Oxidative phosphorylation (enzymes, prosthetic groups, localization in mitochondria, mechanism ATP synthesis)
    6.4) Oxidative stress (reactive oxygen species, their formation, antioxidant enzymes systems, antioxidant molecules)
    7) Lipids
    7.1) Lipids classes (definition, biological) and metabolism
    7.2) Synthesis and degradation of Fatty acids
    7.3) Lipid synthesis. Peroxidation and Eicosanoids.
    7.4 Biotransformation
    8) Nucleic acids and their metabolism
    8.1) Structure of components, role of nucleotides, biosynthesis (replication, transcription and proteosynthesis
    8.2) Nucleic acids metabolism (biosynthesis and degradation of purine and pyrimidine nucleotides)
    9) Integration of metabolism and cell signaling


    Lecturer: M.Sc. Marie Brázdová, Ph.D., Exercising: M.Sc. Denis Šubert M.Sc. Tereza Pavlicová M.Sc. Zuzana Soldánová Dr. Ing. Branislav Ruttkay-Nedecky M.Sc. Marie BRAZDOVÁ, Ph.D. Discipline guarantor: M.Sc. Marie Brázdová, PhD.
Literature
    required literature
  • Josef Tomandl, Eva Táborská a kolektiv. Biochemie I : semináře. Brno, 2012. ISBN 978-80-210-5740-1. info
    recommended literature
  • MURRAY,R.K., GRANNER,D.K.,RODWELL,V.W. Harper´s Illustrated Biochemistry. Appleton & Lange, 2006. ISBN 07-147885-X. info
  • Robert K. Murray, Daryl K. Granner, Peter A. Mayes, Victor W. Ro. Harperova biochemie. ČR, 2002. ISBN 80-7319-013-3. info
  • CHAMPE,P.C.,HARVEY,R.A. Lippincott´s Illustrated Reviews: Biochemistry. Lippincott Wiliams & Wilkins, 1994. ISBN 0-397-51091-8. info
  • MURRAY,R.K., GRANNER,D.K.,MAYES,P.A.,RODWELL,V.W. Harperova biochemie (český překlad). Nakladatelství a vydavatelství H & H, 1993. ISBN 80-85787-38-5. info
  • APPS,D.K.,COHEN,B.B.,STEEL,C.M. Biochemistry (A concise text for medical students). Bailliére Tindall, 1992. ISBN 70-20-1444-3. info
Teaching methods
lectures, class discussion, laboratory work, homework,
Assessment methods
Successful completion of the course with a written and oral exam. The grade depends mainly on the written part, which consists of a test with 40 questions, 150 points. To pass the course successfully, you must pass the test at 60%. The prerequisite for passing the exam is passing all practical exercises, passing the test from the exercises with 80%, adequate knowledge of biochemical issues and correct preparation of all exercise protocols. At the same time, a short task readiness test will be conducted before each exercise. During the course of study, it is mandatory to complete 4 tests from basic biochemical pathways. Furthermore, the group task of developing 2 chapters of seminars on Biochemistry (Josef Tomandl, Eva Táborská and the team. Biochemistry I : seminars. Brno, 2012. ISBN 978-80-210-5740-1). The written examination test is prepared on the basis of lectures and questions from Biochemistry I-seminar 2012 - see recommended literature. He will then make up the teaching at the time and in the manner determined by the relevant teacher.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Student requirements Successful completion of the course with a written and oral exam. The grade depends primarily on the written part of the test. To pass the course successfully, you must pass the test at 60%. Requirements for the student's readiness for practical teaching, conditions for passing the test from the practical part of the teaching. During the semester, students are required to demonstrate their readiness for practical teaching according to the requirements set by the head of the discipline at the beginning of the semester. The prerequisite for mastering the subject "BIOCHEMISTRY" is the completion of all practical exercises, adequate knowledge of biochemical issues and the correct preparation of all exercise protocols. At the same time, a short task readiness test will be conducted before each exercise. During the course of study, it is mandatory to complete 4 tests from basic biochemical pathways. The written test is prepared on the basis of lectures and questions from Biochemistry I-Seminars - see recommended literature (Tomandl et al. 2012). In case of poor-quality preparation, the student will be excluded from the exercise, as it could immediately endanger the course of the exercise or cause harm to the health of any of the participants of the practical training. He will then make up the teaching at the time and in the manner determined by the relevant teacher. If the student fails the test, he/she will take the test on a date set by the relevant practical teacher. Absences from practical classes must be substantiated by valid documents. He is responsible for the technical content of the practical exercises in biochemistry, and the guarantor of the discipline ensures the material security and operation of the practical exercises.
The course is also listed under the following terms Autumn 2020, Autumn 2021, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2022, recent)
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