F1PB1_16 Pathobiochemistry

Faculty of Pharmacy
Spring 2021
Extent and Intensity
2/1/0. 4 credit(s). Type of Completion: zk (examination).
Taught partially online.
Teacher(s)
Mgr. Marie Brázdová, Ph.D. (lecturer)
Mgr. Marek Petr, Ph.D. (lecturer)
Mgr. Marie Brázdová, Ph.D. (seminar tutor)
Mgr. Marek Petr, Ph.D. (seminar tutor)
PharmDr. Zuzana Soldánová (seminar tutor)
Mgr. Magdaléna Drotárová (lecturer)
PharmDr. Zuzana Soldánová (lecturer)
Mgr. Magdaléna Drotárová (seminar tutor)
Mgr. Bc. Daniela Nykodýmová (seminar tutor)
Ing. Branislav Ruttkay-Nedecký, Dr. (seminar tutor)
Guaranteed by
Mgr. Marie Brázdová, Ph.D.
Department of Molecular Pharmacy – Departments – Faculty of Pharmacy
Timetable
Mon 7:15–8:45 posluchárna, -056
  • Timetable of Seminar Groups:
F1PB1_16/01: Tue 30. 3. to Tue 18. 5. each odd Tuesday 7:00–9:15 Laboratoř praktických cvičení, 323
F1PB1_16/02: Tue 30. 3. to Tue 18. 5. each odd Tuesday 9:15–11:30 Laboratoř praktických cvičení, 323
F1PB1_16/03: Tue 30. 3. to Tue 18. 5. each odd Tuesday 11:30–13:45 Laboratoř praktických cvičení, 323
F1PB1_16/04: Tue 30. 3. to Tue 18. 5. each odd Tuesday 11:30–13:45 Laboratoř praktických cvičení, 324
F1PB1_16/05: Tue 30. 3. to Tue 18. 5. each even Tuesday 7:00–9:15 Laboratoř praktických cvičení, 323
F1PB1_16/06: Tue 30. 3. to Tue 18. 5. each even Tuesday 9:15–11:30 Laboratoř praktických cvičení, 323
F1PB1_16/07: Tue 30. 3. to Tue 18. 5. each even Tuesday 11:30–13:45 Laboratoř praktických cvičení, 323
F1PB1_16/08: Tue 30. 3. to Tue 18. 5. each even Tuesday 11:30–13:45 Laboratoř praktických cvičení, 324
Prerequisites
The knowledge of subject in range of lectures
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 96 student(s).
Current registration and enrolment status: enrolled: 58/96, only registered: 0/96, only registered with preference (fields directly associated with the programme): 0/96
fields of study / plans the course is directly associated with
Course objectives
Pathobiochemistry follows the course of Biochemistry taught in the winter semester. Biochemistry deals with the metabolic processes in living organisms.
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy.
The aim of the practical exercises are analyzes of some selected diagnostically important compounds, the correct interpretation of the results of analyzes and their use for the purpose of human diagnostic and therapeutic practice.
Learning outcomes
The aim of the Pathobiochemistry course is to obtain knowledge of the possible disorders of metabolic processes, their causes, symptoms, possible reparations and therapy. Advance knowledge of biochemistry forming the basis for follow-up subjects Methods of molecular biology, Biotechnology of drugs, Exercises from drug biotechnology and Pharmacogenomics.
Syllabus
  • Syllabus Pathobiochemistry

    1. Introduction, the importance of studying pathobiochemistry. The scope and requirements for successful completion of the course exam, recommended literature. Understanding the regulation of metabolism. Biochemical communication.
    2. Metabolism disorders, types and causes. Hereditary metabolic diseases. Enzymes, regulation of metabolism. Causes increased activity of cellular enzymes in the plasma. Clinically significant enzymes.
    3. Pathobiochemistry of carbohydrates, glucose metabolism and its disorders. Glycemic control disorders. Pathobiochemistry of diabetes mellitus, types of DM. Disorders of glycogen metabolism, glykogenosis.
    4. Amino acid metabolism and its disorders. Types of diseases and therapy.
    5. Disorders of lipid metabolism. Cholesterol, lipoproteins. Lipidosy, dyslipoproteinaemia.
    6. Pathobiochemistry of arteriosclerosis. Ischemic heart failure - cardiac markers.
    7. The nucleic acid metabolism disorders of purine and pyrimidine. Hyperuricemia, orotacidurie, therapy.
    8. Blood, blood plasma proteins. Blood clotting, coagulopathy. Dysproteinaemias. Porphyrins. Biosynthesis, metabolism disorders. Porphyria, hemoglobinopathies.
    9. Xenobiotics and their effects on the body. Detoxification mechanism. Biological oxidation. The effects of free radicals on the organism. Lipoperoxidation antioxidants.
    10. Tumor, tumor markers. Basic characteristics of tumor cells. Strategy laboratory tests. Requirements ideal tumor marker. Used tumor markers.
    11. Relation between Pathobiochemistry and Clinical Biochemistry. Clinical and biochemical analysis and its specific features. Terminology of Clinical Biochemistry. The analyzed material. Material removal.
    12. Analysis of urea and the urinary sediment. Immunochemical methods.
    13. Hormones and neurodegenerative disorders.
    Sylabus of practical exercises in pathobiochemistry:
    .1. Exercise: DETERMINATION OF THE CONCENTRATION OF TOTAL PROTEINS IN A SERUM SAMPLE USING AN AUTOMATED PHOTOMETRIC ANALYZER BS 200 2. Exercise: Analysis of a tumor suppressor by immunodetection on a membrane. 3. Exercise: Functional characterization of biomarkers - viability testing, (theoretical) 4. Exercise: Validation analyzes - Analysis of biomarker expression using qRT-PCR,
    5. Exercise: Final test
    Guarantors: Mgr. Marie Brázdová, Ph.D.,
    Tutorial lecturers:
    Mgr. Marie BRÁZDOVÁ, Ph.D.
    , Mgr. M. Peter, Mgr. M.Drotárová, PharmDr. Z.Soldánová
    Lecturers:
    Mgr. Marie BRÁZDOVÁ, Ph.D.
    Mgr. Z. Soldánová, Mgr. M.Drotárová
    Garant of discipline:
    Mgr. Marie Brázdová, PhD.

Literature
    required literature
  • G.F,.Hoffmann et al. Dědičné metabolické poruchy. Grada,, 2012. info
    recommended literature
  • J.Koolman, K.H.Rohm. Barevný atlas biochemie. Grada, 2012. info
  • Murray et al. Harpeŕ´s Illustrated Biochemistry. 29th Edition. Lange, 2012. info
  • Robbins SL, Cotrans RS, Kumar V. The Pathologic Basis of Disease, 3rd Edition. Saunders, 2010. info
  • Ledvina a kol. Biochemie pro studující medicíny - I. a II.díl. Karolinum, 2005. info
  • KARLSON, P., GEROK, W., GROSS, W. Pathobiochemie. Academia, Praha, 1987. info
Teaching methods
lectures, class discussion, laboratory work, homework,
Assessment methods
The written part (online), which consists of a test with 40 questions, 150 points. To successfully complete the course, it is necessary to pass the test at 60%. Prerequisite for passing the exam is obtaining credit, to obtain credit from the subject "PATHBIOCHEMISTRY" it is necessary to complete all practical exercises, passing a credit test at 80%, adequate knowledge of biochemical issues and the correct elaboration of all protocols for exercises. At the same time, a short task readiness test will be performed before each exercise. Furthermore, the group task of elaborating 2 chapters of seminars. The written exam is prepared on the basis of lectures and questions from the Seminars - see recommended literature. He will then reimburse the teaching at the time and in the manner determined by the respective teacher. If the credit is not awarded, the student will perform the credit procedure in the term determined by the relevant teacher of practical training. If the prescribed completion of the course is only a credit, the number of terms for obtaining it is identical, as if the course was completed by an exam.
Language of instruction
Czech
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
Teacher's information
Prerequisite for granting credit from the subject Pathobiochemistry is the completion of all practical exercises and appropriate knowledge of exercises and lectures. Knowledge control is performed in writing (online).
The course is also listed under the following terms Spring 2020, Spring 2023, Spring 2024.
  • Enrolment Statistics (Spring 2021, recent)
  • Permalink: https://is.muni.cz/course/pharm/spring2021/F1PB1_16