PřF:Bi7410 Protein Engineering - Course Information
Bi7410 Protein EngineeringFaculty of Science
- Extent and Intensity
- 1/0/0. 1 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
- doc. Mgr. Radka Chaloupková, Ph.D. (lecturer)
Mgr. David Bednář, Ph.D. (lecturer)
- prof. Mgr. Jiří Damborský, Dr.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: doc. Mgr. Radka Chaloupková, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
- Course Enrolment Limitations
- The course is offered to students of any study field.
- Course objectives
- The aim of this course is to introduce methods and strategies commonly used in protein engineering.
At the end of the course, students should be able to understand and explain differences between rational design and directed evolution, and have a general knowledge about miscellaneous topics such as searches in bioinformatics databases, isolation, expression or purification of novel proteins. Students will also get an overview of several biophysical techniques used for analysis of secondary, tertiary and quaternary structure, as well as of screening methods used for selection of novel protein variants with improved properties.
- 1. Protein synthesis, protein structure, protein function and structure-function relationships.
- 2. Identification of putative enzymes in sequence databases, bioinformatic analysis.
- 3. Isolation of genes from host organisms, cloning, preparation of recombinant proteins, host organisms, protein expression and protein purification.
- 4. Structural characterization of proteins, an overview of spectroscopic techniques for the analysis of protein secondary and tertiary structure; an overview of techniques for analysis of protein quaternary structure.
- 5. Enzymes, enzyme catalysis, factors influencing the speed of enzymatic reaction.
- 6. Enzyme applications, targets of protein engineering, protein engineering approaches, advantages and limitations.
- 7. Rational design, prediction of the structure of enzyme variant, evaluation of the effect of mutations on enzyme structure and function.
- 8. Directed evolution, screening of mutants.
- 9. Examples of application of protein engineering to improve enzyme catalytic efficiency.
- 10. Examples of application of protein engineering to improve enzyme stability.
- 11. Examples of application of protein engineering to improve enzyme enantioselectivity.
- recommended literature
- Protein engineering handbook. Edited by Stefan Lutz - Uwe Bornscheuer. Weinheim: Wiley-VCH, 2009. xli, 409-9. ISBN 9783527318506. info
- not specified
- Directed evolution library creation : methods and protocols. Edited by Frances Hamilton Arnold - George Georgiou. Totowa, N.J.: Humana Press, 2003. x, 224. ISBN 1588292851. info
- FERSHT, Alan. Structure and mechanism in protein science :a guide to enzyme catalysis and protein folding. New York: W.H. Freeman, 1998. xxi, 631 s. ISBN 0-7167-3268-8. info
- Teaching methods
- Lectures, class discussion.
- Assessment methods
- Final written test consists of 25 questions and is scored on a 25-point scale. A minimum score of 13 is required to successfully pass the exam.
- Language of instruction
- Further Comments
- The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (recent)
- Permalink: https://is.muni.cz/course/sci/spring2019/Bi7410