Bi7054 Laboratory Animals in Experimental Biology and Medicine

Faculty of Science
Autumn 2025
Extent and Intensity
1/0/0. 1 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
RNDr. Jiří Salát, Ph.D. (lecturer)
prof. RNDr. Marcela Buchtová, Ph.D. (lecturer)
Mgr. Jakub Harnoš, Ph.D. (lecturer)
Mgr. Nikodém Zezula, Ph.D. (lecturer)
RNDr. Petra Kolísková, Ph.D. (lecturer)
Mgr. Pavel Dobeš, Ph.D. (lecturer)
Mgr. Jana Hurychová, Ph.D. (lecturer)
doc. RNDr. Martin Vácha, Ph.D. (lecturer)
doc. PharmDr. Jana Rudá, Ph.D. (lecturer)
Guaranteed by
doc. RNDr. Martin Vácha, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Martin Vácha, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Fri 12:00–13:50 D36/215
Prerequisites
NOW( Bi7054c Lab Animals in Biol - pr )
The course forms an integral part of the course Laboratory Animals in Experimental Biology and Medicine - Exercise (Bi7054c).
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 8/16, only registered: 0/16
fields of study / plans the course is directly associated with
Course objectives
The aim of the course is to acquaint students with the possibilities of using laboratory animals in research in accordance with current legislation. In addition, in conjunction with the Bi7054c course, to provide practical skills for working with laboratory animals from handling to designing an experimental plan.
Learning outcomes
Graduates of the course will gain information about the use of laboratory animals in research. They will be familiar with the legal regulations that allow and define the use of animals in experiments. They will learn practical information on the breeding and use of animals in research by means of representatives of basic model animal groups.
Syllabus
1) Introduction to the structure of the course, conditions for completion. Introduction of invertebrate models (Drosophila, Caenorhabditis, Galleria). Their development cycle, breeding and handling. 2) Introduction of the model fish group. Development cycle, rearing and handling of Danio rerio embryos. 3) Introduction of the model group of frogs. Development cycle, induction and egg retrieval, dissection and testicular collection, rearing and handling of Xenopus laevis. 4) Introduction of model groups of reptiles and birds. Development, breeding, handling, embryo collection and dissection in Gallus and Chamaeleo. 5) Introduction of the mammal model group. Rearing, handling, tissue collection, dissection, anaesthesia, injection on Mus muscullus. 6) Theoretical background: - Ethical and economic aspects of animal experimentation, 3R principle; - - - - Biological experimental models: laboratory animals, alternative models, factors affecting the quality of life of animals, Consumption of experimental animals in the Czech Republic, Experiments on farm and wild animals; - Genetics of laboratory animals (inbreeding, congenic strains, GMO strains), SPF and barrier breeding; - Biology and physiology of laboratory animals; - Diseases of laboratory animals and diagnostic methods, Basics of zoohygiene; - Experimental procedures with laboratory animals (handling, collection of biological material, administration of substances, anaesthesia, euthanasia); - Animal models of human diseases, Humanized mice; - Legislation allowing and defining the use of animals in experiments; Law for the Protection of Animals against Cruelty; Ordinance on the Protection of Experimental Animals - Designing animal experiments, Plan of the experiment. Consultation of the actual project of the experiment.
Literature
    recommended literature
  • Zákon č. 246/1992 Sb. na ochranu zvířat proti týrání
  • Ochrana, chov a využití pokusných zvířat. Edited by Lukáš Jebavý. Brno: Společnost pro vědu o laboratorních zvířatech, 2014, 454 s. ISBN 9788021324862. info
  • HEDRICH, H.J. The Laboratory Mouse. 2nd ed. Academic Press, 2012. ISBN 978-0-12-382008-2. info
Teaching methods
Teaching is in the form of a lecture accompanied by a projected presentation, every other week in a 4-hour block in conjunction with the practical course Bi7054c.
Assessment methods
The project of the experiment, which the student presents and defends in discussion with the teachers during the exam, will be evaluated.
Language of instruction
Czech
Further Comments
Study Materials
The course is taught annually.
Listed among pre-requisites of other courses
  • Enrolment Statistics (recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2025/Bi7054