Bi8110 Mechanisms of carcinogenesis

Faculty of Science
Spring 2026
Extent and Intensity
2/0/0. 2 credit(s) (plus extra credits for completion). Type of Completion: zk (examination).
In-person direct teaching
Teacher(s)
RNDr. Vendula Hlaváčková Pospíchalová, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
doc. Mgr. Karel Souček, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Karel Souček, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology – Biology Section – Faculty of Science
Prerequisites
Bi7070 Physiology of Cell Systems
Basic knowledge of biochemistry, cell physiology and molecular biology and passing through lecture of Physiology of cell systems are neccessary.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 9 fields of study the course is directly associated with, display
Course objectives
The course gives a complex view of the process of carcinogenesis, including mutagenic (genotoxic), epigenetic, and non-genotoxic factors that play a role during the development of cancer. Molecular and biological principles of changes in extra-, inter-, and intracellular communication leading to disruption of cellular proliferation, differentiation, and apoptosis and the role of stem cells, specific signalling pathways, and tumour microenvironment in cancer development are explained. The students obtain knowledge of development of frequent cancers such as leukemias, colon, prostate, breast, lung, liver, and skin cancer including specific mechanisms. Next, the role of environmental factors, especially dietary lipids and lipid metabolism in carcinogenesis is explained. Finally, possibilities and importance of recent diagnostic and therapeutic approaches including mechanisms of action of specific drugs are described.
Learning outcomes
At the end of the course students should be able to: understand a complex view of the process of carcinogenesis, including mutagenic (genotoxic), epigenetic, and non-genotoxic factors that play a role during the development of cancer; explain and understand basic processes leading to cancer development including metastases on the level of cells and cellular polupations; interpret molecular and biological principles influencing extra-, inter-, and intracellular communication and specific signal transduction leading to disruption of cellular proliferation, differentiation, and apoptosis; explain the role and importance of stem cells, specific signalling pathways and tumour microenvironment in cancer development; describe mechanisms of frequent cancer type development (leukemias, colon, prostate, breast, lung, liver, and skin cancer); understand mechanisms of the effects of environmental factors, especially dietary lipids and lipid metabolism in carcinogenesis; discuss questions of cancer prevention, diagnosis and therapy; use acquired knowledge of new research results from described areas for prevention and new therapeutic strategies.
Syllabus
Cancer origin and development; history, terminology, basic concepts. Individual cancer stages (initiation, promotion, progression) and their characteristic; genetic, epigenetic and non-genotoxic aspects; Cell transformation, cell contact inhibition; Type of tumours; spontaneous and inducible tumours (oncogenic viruses, chemical and physical carcinogens), benign and malignant tumours, classification according to tissues. Importance of mutations in cancer development; DNA damage and repair; microsatellites, genetic instability; clonal selection and expansion; gene amplification, loss of heterozygosity; cell cycle control, apoptosis and mutated phenotype; genetic vs. environmental factors; human hereditary syndromes. Molecular basis of cancer development; proto-oncogenes and oncogenes, tumour suppressor genes; viral transformation; activation and inactivation mutations; growth stimulation and inhibition; autocrine signalling; feedback regulation. Epigenetic mechanisms of carcinogenesis; DNA methylation, histone acetylation, microRNA. Cell immortalization; telomeres and telomerase, factors affecting immortalization, use in cancer therapy. Cancer promotion; non-genotoxic factors and mechanisms; the role of cellular communication and interaction; stimulatory and inhibitory signals; growth regulators in carcinogenesis; the role and importance of gap junctions (GJ); regulation of GJ and its disturbances in cancer; deregulation of proliferation, differentiation and apoptosis; tissue homeostasis. Hormone-dependent cancers; signal transduction of steroid hormones, intracellular receptors; exogenous hormones. Cancer stem cell theory; the role in cancer development, tumour heterogeneity. The role of tumour microenvironment; specific signalling and cell communication. Metastases and their origin; metastatic cascade, modulation of cell-cell and cell-extracellular matrix contacts; changes of adhesive molecules and intracellular signalling; disturbance of proteolytic-antiproteolytic balance, the role of mucins. Epithelial-mesenchymal transition and its regulation. Angiogenesis; its significance; interaction of cancer cell with endothelium; the role of cell adhesion; inducers and inhibitors of angiogenesis. Development of specific cancer types (genetic and epigenetic causes, environmental factors); Haemopoietic disorders - leukemias; chronic myeloid leukemia, myelodysplastic syndrome, acute leukemia, genetic disorders, disturbing of proliferation, differentiation, and apoptosis; diagnosis and therapy; therapeutic use of cytokines. Epithelial tumours; carcinomas of colorectum, breast, prostate and skin; other cancer types; Genetic and non-genetic causes; hereditary syndromes; precancerous stages, ethiology, development, specific signalling pathways; endogenous factors – cytokines, growth factors, eicosanoids; the role of environmental factors. Hormone-dependent cancers; steroid hormone signal transduction; intracellular receptors; exogenous hormones. Environmental factors in carcinogenesis; radiation; chemical carcinogens, viruses, bacteria; Significance of life style; Nutritional aspects of carcinogenesis; Food carcinogens, promotional and antipromotional effects of dietary compounds (vitamins, antioxidants). Content and composition of dietary fat; polyunsaturated fatty acids (PUFAs, n-6 and n-3 types) and their metabolites in carcinogenesis; molecular mechanism of their action; modulation of cell membranes and cellular lipidome, oxidative metabolism; fibre and short-chain fatty acids (butyrate). Practical aspects, prevention, clinics. Cancer prevention, diagnostics and therapy; Preventive factors; Tumour diagnosis and specific markers; prognostic vs. predictive factors; cytokinetic parameters, molecular and other markers; Predictive oncology, molecular pathology; Modern detection methods (flow and high resolution cytometry and microscopy, laser scanning, molecular methods, microarrays); Therapy - surgery, radiation, chemotherapy, immunotherapy; supporting factors. Mechanisms of action of chemotherapeutics; mechanisms of drug resistance; New trends in cancer therapy; comparison of methods and interpretation of measured parameters; Data evaluation, bioinformatics, multivariate analyses.
Literature
    recommended literature
  • The Biology of Cancer, ISE – International Student Edition, 3rd Edition Autor Robert A. Weinberg
  • Molecular Biology of the Cell International Student Edition Bruce Alberts Nakladatel W. W. Norton & Company, 2022
Teaching methods
Lectures and class discussion. Learning answers for individual lectures in e-learning didacting material.
Assessment methods
The course is finished by written exam.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course is taught annually.
The course is taught every week.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms Spring 2008 - for the purpose of the accreditation, Spring 2011 - only for the accreditation, Spring 2003, Spring 2004, Spring 2005, Spring 2006, Spring 2007, Spring 2008, Spring 2009, Spring 2010, Spring 2011, Spring 2012, spring 2012 - acreditation, Spring 2013, Spring 2014, Spring 2015, Spring 2016, Spring 2017, spring 2018, Spring 2019, Spring 2020, Spring 2021, Spring 2022, Spring 2023, Spring 2024, Spring 2025.
  • Enrolment Statistics (Spring 2026, recent)
  • Permalink: https://is.muni.cz/course/sci/spring2026/Bi8110