B5491 Molecular Biology - Practice 1

Faculty of Science
Autumn 2001
Extent and Intensity
0/8/0. 9 credit(s). Type of Completion: graded credit.
Teacher(s)
prof. RNDr. Roman Pantůček, Ph.D. (seminar tutor)
doc. RNDr. Vladislava Růžičková, CSc. (seminar tutor)
prof. RNDr. Jan Šmarda, CSc. (seminar tutor)
Guaranteed by
doc. RNDr. Vladislava Růžičková, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Vladislava Růžičková, CSc.
Prerequisites
SOUHLAS || B4070 General mikrobiology || B4090 General microbiology || B4090p General microbiology -l || BX090 || BMB22 General microbiology || B2050 General microbiology
Molecular structure reproduction a function of procaryotic and eucaryotic genome. Cultiovation of bacteria, estimation of titre, CFU.
Course Enrolment Limitations
The course is only offered to the students of the study fields the course is directly associated with.

The capacity limit for the course is 16 student(s).
Current registration and enrolment status: enrolled: 0/16, only registered: 0/16
fields of study / plans the course is directly associated with
Course objectives
The subject is a part of basic course Molecular biology for students of discipline Molecular and cellular diagnostic. It comprises the tasks that offer acquiring of basic methods in molecular biology of bacteria and their viruses.
Syllabus
  • Preparation of solutions used in molecular biology. Isolation spontaneous mutants of Staphylococcus aureus resistant to streptomycine. One step growth curve of bacteriophage. Fluctuation test. Replica-plating of colonies a plaques. Isolation of plasmid DNA; Determination purity and concentration of DNA; Digestion of DNA by restriction endonucleases; Horizontal slab gel electrophoresis of DNA in agarose gels; Cultivation of eucaryotic cell cultures. Cytocentrifugation of influenced eucaryotic cells; Isolation of proteins; SDS-page methods.
Literature
  • Návody k úlohám jsou dostupné u vyučujících
  • MILLER, Jeffrey H. A Short course in bacterial genetics : a laboratory manual and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press, 1992, 456 s. ISBN 0879693495. info
  • SAMBROOK, J., E.F. FRITSCH and T. MANIATIS. Molecular Cloning. A laboratory Manual. Second Edition. Cold Spring Harbor: Cold Spring Harbor Laboratory Press, 1989. ISBN 0-87969-309-6. info
Assessment methods (in Czech)
Výuka: praktické cvičení, zápočet: písemné testové otázky, protokoly.
Language of instruction
Czech
Follow-Up Courses
Further comments (probably available only in Czech)
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: in blocks.
The course is also listed under the following terms Autumn 1999, Autumn 2000.
  • Enrolment Statistics (recent)
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