Bi7270 Plant Cytology and Anatomy for Advanced Students

Faculty of Science
Autumn 2003
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
RNDr. Jaroslava Dubová, CSc. (lecturer)
Guaranteed by
RNDr. Jaroslava Dubová, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Eliška Karasová
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 5 student(s).
Current registration and enrolment status: enrolled: 0/5, only registered: 0/5, only registered with preference (fields directly associated with the programme): 0/5
fields of study / plans the course is directly associated with
Course objectives
Light Microscope Construction and Function (Bright Field, Phase Contract Microscopy, Interference-DIC). Replicas for the Study of Surface. Rapid Cytological Methods: Cell Cycle, Nuclear Staining. Embryology: Microsporogenesis, Pollen Grain Development and Structure, Megasporogenesis, Embryo Sac,Fertilisation, Zygote, Embryo and Endosperm Development, Whole Mounts of Cleared Preparations. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. Cryosections and Histochemistry. Fluorescence Microscopy. Documentation.
Syllabus
  • 1. Development of Light Microscope Construction and Function (Bright Field, Köhler Illumination). 2. Replicas for the Study of Surface, Epidermis, Stoma Cells, Subsidiary Cells, Types of Stoma Structure. 3. Rapid Cytological Methods: Meristems, Cell Cycle, Cytoskeleton Organization during Cell Cycle, Nuclear Staining (Lacto-propionic Orcein, Cajal-Brožek Method), Pre-treatment. 4. Androeceum: Stamen, Development and Structure of Anther, Microsporogenesis, Pollen Grain Development and Structure, Pollen Grain Germination, Pollen Grain Viability. 5. Gynaecium: Whole Mounts of Cleared Ovules and Seeds, Megasporogenesis, Embryo and Endosperm Development. Seed Structure. 6. Phase Contract Microscopy, Nomarski DIC. 7. Histological Methods: Fixation, Dehydration, Infiltration and Embedding, Sectioning, Dyes and Staining. 8. Cryosections and Enzyme Histochemistry. 9. Fluorescence Microscopy, Autofluorescence, Fluorochromes, Immunofluorescence. 10. Documentation: Photomicrography, Video, and Distance Measurements.
Literature
  • PAZOURKOVÁ, Zdeňka. Botanická mikrotechnika. 1. vyd. Praha: Univerzita Karlova, 1982, 166 s. info
  • NĚMEC, Bohumil and A KOL. Botanická mikrotechnika (Plant Microtechnique). Praha: ČSAV, 1962, 484 pp. ISBN 21-103-62. info
  • KNOZ, Jan and Věra OPRAVILOVÁ. Základy mikroskopické techniky. 1. vyd. Brno: Masarykova univerzita, 1992, 195 s. ISBN 8021004738. info
  • PAZOUREK, Jaroslav and Olga VOTRUBOVÁ. Atlas of plant anatomy. Prague: PERES Publishers, 1997, 447 s. ISBN 80-901691-2-0. info
  • ESAU, Katherine. Anatomy of seed plants. 2nd ed. New York: John Wiley & Sons, 1977, xx, 550. ISBN 0471245208. info
  • ERDELSKÁ, Olga. Embryológia krytosemenných rastlín (Angiosperm embryology). Bratislava: Veda, 1981, 200 pp. ISBN 71-020-81. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum I (Plant Anatomy Practice I). 4.Ed. Jena: VEB Gustav Fischer Verlag, 1983, 279 pp. ISBN 261 700-38-83. info
  • BRAUNE, Wolfram, Alfred LEMAN and Hans TAUBERT. Pflanzenanatomisches Praktikum II (Plant Anatomy Practice II). 2nd ed. Jena: VEB Gustav Fischer Verlag, 1982, 426 pp. ISBN 261 700-36-82. info
  • Staining procedures. Edited by George Clark. 4th ed. Baltimore: Williams and Wilkins, 1981, xi, 512 s. ISBN 0-683-01707-1. info
  • KIERNAN, J. Histological and histochemical methods: Theory and practise. 1.Ed. Oxford: Pergamon Press, 1981, 344 pp. info
  • Histochemistry :theoretical and applied. Edited by Peter J. Stoward - A. G. Everson Pearse. 4th ed. Edinburgh: Churchill Livingstone, 1991, xiv, 728 s. ISBN 0-443-02996-2. info
  • HAWES, Chris and Béatrice SATIAT-JEUNEMAITRE. Plant Cell Biology. Oxford: University Press, 2001, 338 pp. Practical Approach. ISBN 0-19-963865-9. info
  • FOSKET, Donald E. Plant growth and development : a molecular approach. San Diego: Academic Press, 1994, xix, 580 s. ISBN 0-12-262430-0. info
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2003, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2003/Bi7270