Bi7430 Molecular biotechnology

Faculty of Science
Autumn 2004
Extent and Intensity
2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Ing. Bohuslav Rittich, CSc. (lecturer)
doc. RNDr. Alena Španová, CSc. (lecturer)
Guaranteed by
doc. Ing. Bohuslav Rittich, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Timetable
Tue 11:00–12:50 Bpt,01013
Prerequisites
Výuka je určena zájemcům o obor molekulární biotechnologie. Předpokladem zapsání předmětu je zkouška z předmětu Molekulární biologie(Bi4020) a Obecná mikrobiologie (Bi4090).
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
Course objectives
Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creating and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotores. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Directed mutagenesis and protein engineering. Production of compounds using genetically modificated microorganisms (GMO).Enzymic catalysis in anhydrous solvents. Determination of identity and the purity of recombinant proteins. Regulation of the use of GMO.
Syllabus
  • Relationship between chemical production and biotechnologies. Molecular biotechnology. Preparation of recombinant DNA molecules. Use of cloning vectors. Artificial gene transfer. Selection of cells with recombinant DNA. Amplification of foreign DNA. Creation and screening of genomic libraries. Chemical synthesis of DNA and peptides.DNA chips production. Manipulation of gene expression. Isolation of functional promotors. Regulatable promotors. Fusion proteins. Translation expression vectors. Increasing of protein stability. Characterisation of microorganisms used for heterologous protein production. Direct mutagenesis and protein engineering. Production of compounds using genetically modified microorganisms (GMO). Enzymatic catalysis in anhydrous solvents. Determination of identity and purity of recombinant proteins. Regulations of the use of GMO.
Literature
  • 1. Watson J.D., Gilman M., Witkowski J. Zoller M. Recombinant DNA (2nd ed.), SAB, New York 1992. 2. Glick B.R., Pasternak J.J. Molecular Biotechnology, ASM Press, Washington 1994.
Assessment methods (in Czech)
Předmět je ukončen zkouškou
Language of instruction
Czech
Follow-Up Courses
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2004, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2004/Bi7430