Bi9250c Special Immunological Methods - practical course

Faculty of Science
Autumn 2005
Extent and Intensity
0/2/0. 2 credit(s). Recommended Type of Completion: z (credit). Other types of completion: graded credit.
Teacher(s)
doc. RNDr. Alena Žákovská, Ph.D. (seminar tutor)
Guaranteed by
doc. RNDr. Alena Žákovská, Ph.D.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Alena Žákovská, Ph.D.
Prerequisites
This practice shall inform students in a practical form about chosen immunological methods used in immunology. The practice concerns ELISA, electrophoresis PAGGE and WB methods.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 12 student(s).
Current registration and enrolment status: enrolled: 0/12, only registered: 0/12, only registered with preference (fields directly associated with the programme): 0/12
fields of study / plans the course is directly associated with
  • General Biology (programme PřF, M-BI, specialization Physiology of Animals)
  • General Biology (programme PřF, N-BI, specialization Physiology of Animals)
Course objectives
Chosen laboratory methods: ELISA, electrophoresis PAGGE, Western Blot
Syllabus
  • The practice proceeds in the form of full-time work during 4 days, in which individual parts of methods are solved: 1. putting of antigen on the surface of microtitration plates and their preparation for ELISA test 2. testing on the presence of antibodies against chosen pathogen in serum 3. preparation of acrylamid gel for PAGGE method 4. electrophoretic separation of protein fractions of pathogen 5. transmission of protein fractions from the gel to nitrocelulose membrane by Western Blot method 6. detection of serum antibodies against proteins on membrane
Language of instruction
Czech
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
Listed among pre-requisites of other courses
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023, Autumn 2024.
  • Enrolment Statistics (Autumn 2005, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2005/Bi9250c