C8857 Study of interactions between proteins and DNA

Faculty of Science
Autumn 2007
Extent and Intensity
1/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Teacher(s)
doc. Mgr. Lumír Krejčí, Ph.D. (lecturer)
Guaranteed by
doc. Mgr. Lumír Krejčí, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 15 fields of study the course is directly associated with, display
Syllabus
  • 1) General concept DNA RNA PROTEIN; Proteins - their structure, assmebly, and folding; Mad cow and other disease; Domains and motifs; Predictions. 2) Why and how do the proteins interact; Little bit of Math; Data mining; Protein localization (NLS), membrane transport , transport in cytosol etc.); Role of protein interactions (cell cycle, enviroment and hormon responses). 3) Modification of protein interactions, their role and types (phosphorylation, Ubq, Sumo, glycosilation, 4) DNA-protein interactions, types of interaction motivs, their roles (replication, transcription, repair), modification again 5) Methods of study (microscopy, Y2H, FRET, in vitro /pulldow). 6) Methods II (IP,CHIP, far western, EMSA, Biocore etc.). 7) Single protein versus complex of proteins. 8) Examples: DNA repair mechanisms, from single protein to multi-protein complexes. 9) DNA repair II
Language of instruction
English
Further Comments
Study Materials
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, Autumn 2019, Autumn 2020, autumn 2021, Autumn 2022, Autumn 2023.
  • Enrolment Statistics (Autumn 2007, recent)
  • Permalink: https://is.muni.cz/course/sci/autumn2007/C8857