PřF:Bi7030c Bacterial physiology - pr. - Course Information
Bi7030c Bacterial physiology - practical course
Faculty of ScienceAutumn 2008
- Extent and Intensity
- 0/5/0. 5 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. Ing. Martin Krsek, CSc., MSc (seminar tutor)
- Guaranteed by
- doc. RNDr. Miroslav Němec, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Němec, CSc. - Timetable
- Wed 8:00–12:50 Bpb,02012
- Timetable of Seminar Groups:
Bi7030c/02: No timetable has been entered into IS. M. Krsek - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Molecular Biology and Genetics (programme PřF, B-BI)
- Molecular Biology and Genetics (programme PřF, M-BI)
- General Biology (programme PřF, M-BI, specialization Microbiology)
- General Biology (programme PřF, N-BI, specialization Microbiology)
- Course objectives
- Main objective of the course is to introduce students to principals of work with bacterial cells and bacteriophages and presenting them with a set of chosen tasks to equip them with skills needed for solution of various problems they can face in bacterial physiology. Using set of chosen techniques used in physiological laboratories they will familiarize themselves with growth characteristics of bacterial cells and phages, influence of temperature and UV on lysogenic cells and phages, they will prepare crude enzymatic preparation and test its enzymatic activity.
- Syllabus
- Determination of the bacterial proteins and dry-weight. Bacterial growth curve in static culture (using plate counts). One-step growth curve of the polyvalent and virulent staphylopage. Determination of dehydrogenase activity. Preparation of the crude enzymatic preparation (HEP) with phosphatase activity. Determination of phosphatase activity of native cells and HEP. Oxygen consumption (Oxi-Top). Biodegradation. Influence of heavy metals on bacterial growth (Bioscreen).
- Literature
- Horáková, D., Němec, M. (2003) : Laboratorní cvičení z fyziologie bakterií
- Schemata pro jednotlivé úlohy poskytne vyučující
- Assessment methods
- Practical course, reports, credit.
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught annually. - Listed among pre-requisites of other courses
- Enrolment Statistics (Autumn 2008, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2008/Bi7030c