PřF:Bi7016 Nucleic acids chemistry-pract. - Course Information
Bi7016 Nucleic acids chemistry - lab. practice
Faculty of ScienceAutumn 2010 - only for the accreditation
- Extent and Intensity
- 0/1/0. 1 credit(s). Type of Completion: z (credit).
- Teacher(s)
- doc. RNDr. Miroslav Fojta, CSc. (seminar tutor)
prof. Mgr. Václav Brázda, Ph.D. (assistant)
Mgr. Marie Brázdová, Ph.D. (assistant)
Mgr. Hana Pivoňková, Ph.D. (assistant) - Guaranteed by
- prof. RNDr. Jan Šmarda, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: doc. RNDr. Miroslav Fojta, CSc. - Prerequisites
- NOW ( Bi7015 Nucleic acids chemistry )
Previous or parallel passing-out the Bi7015 subject - Course Enrolment Limitations
- The course is also offered to the students of the fields other than those the course is directly associated with.
- fields of study / plans the course is directly associated with
- Biochemistry (programme PřF, M-CH)
- Biochemistry (programme PřF, N-CH)
- Molecular Biology and Genetics (programme PřF, M-BI)
- Molecular Biology and Genetics (programme PřF, N-BI)
- Course objectives
- At the end of the course students will be trained to perform simple experiments based on methods and experimental techniques that are related to topics of the course Bi7015.
- Syllabus
- 1. Gel electrophoresis of nucleic acids, proteins and their complexes, two-dimesional electrophoresis. 2. Blotting, protein immunodetection. 3. Magnatoseparation techniques, immunoprecipitation. 4. Centrifugation methods, DNA precipitation, extraction with organic solvents, gradient centrifugation, ultrafiltration. 5. Chromatographic methods, FPLC of proteins on ionex, affinity and gel-filtration columns. 7. DNA sequencing and footprinting. 7. Electrochemical methods, determination of DNA and proteins, detection of DNA damage and interactions.
- Literature
- BLACKBURN, M. G. and M. J. GAIT. Nucleic Acids in Chemistry and Biology. Oxford: Oxford University Press, 1996. info
- SINDEN, Richard R. DNA structure and function. San Diego: Academic Press, 1994, xxiii, 398. ISBN 0126457506. info
- ADAMS, R. L. P., J. T. KNOWLER and D. P. LEADER. The Biochemistry of Nucleic Acids. 10th edit. London: Chapman and Hall, 1986. info
- Teaching methods
- Practical courses, demonstrations in the laboratory
- Assessment methods
- Teaching method: practical demonstrations of instruments and real experimental data, simple experiments conducted by the students Type of exam: credit (given on the basis of participance)
- Language of instruction
- Czech
- Further comments (probably available only in Czech)
- The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
- Enrolment Statistics (Autumn 2010 - only for the accreditation, recent)
- Permalink: https://is.muni.cz/course/sci/autumn2010-onlyfortheaccreditation/Bi7016