C7999 Advanced Methods of NMR Spectroscopy

Faculty of Science
Autumn 2010 - only for the accreditation
Extent and Intensity
0/0/2. 2 credit(s) (plus 1 credit for an exam). Recommended Type of Completion: zk (examination). Other types of completion: k (colloquium).
Teacher(s)
prof. RNDr. Radek Marek, Ph.D. (seminar tutor)
Mgr. Jan Vícha, Ph.D. (seminar tutor)
Guaranteed by
prof. RNDr. Radek Marek, Ph.D.
National Centre for Biomolecular Research – Faculty of Science
Contact Person: prof. RNDr. Radek Marek, Ph.D.
Prerequisites
Students should have knowledge of NMR at the level of the courses: NMR Structural Analysis C8950 or Theoretical Concepts of NMR C5320
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
The capacity limit for the course is 3 student(s).
Current registration and enrolment status: enrolled: 0/3, only registered: 0/3, only registered with preference (fields directly associated with the programme): 0/3
fields of study / plans the course is directly associated with
Course objectives
At the end of the course students should be able to develop advanced methods of 1D and 2D NMR spectroscopy of small molecules. The course is focused on hands-on experience of modern techniques in small molecule NMR spectroscopy.
Syllabus
  • 1. Basic 1D NMR spectra: pulse sequence, acquisition parameters, pulse calibration (direct and indirect), probehead exchange 2. Processing and parameters, temperature measurement 3. Homonuclear 2D chemical shift correlation experiments: 1H-1H COSY, 31P-31P COSY, MQF-COSY, NOESY 4. Heteronuclear 2D chemical shift correlation experiments: 31P-19F HETCOR, COLOC 5. Inverse (proton) detection: HSQC, HMQC, HMBC, pulsed-field gradients 6. Practical aspects for measuring various nuclei: 15N nucleus (direct and indirect detection), nucleus with spin > 1/2 (14N, 17O), 29Si, 77Se, 195Pt 7. Selective and shaped pulses, combined (hybrid) experiments: selective NOE, HSQC-TOCSY
Literature
    recommended literature
  • Claridge, Timothy D.W. High-Resolution NMR Techniques in Organic Chemistry, Amsterdam, Pergamon, 1999, ISBN 0-08-042798-7
  • Braun, S. - Kalinowski, H.O. - Berger, S. 100 and More Basic NMR Experiments, Weinheim, VCH, 1996, ISBN 3-527-29091-5
Teaching methods
Laboratory training and class discussion.
Assessment methods
Presence in practical sessions in NMR laboratory is required. Final examination will be based on the structural analysis of unknown sample by using 2D NMR techniques.
Language of instruction
English
Further Comments
The course can also be completed outside the examination period.
The course is taught annually.
The course is taught: every week.
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2002, Autumn 2003, Autumn 2004, Autumn 2005, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012.