PřF:Bi9393 Analytical cytometry - Course Information

Bi9393 Analytical cytometry

Extent and Intensity

2/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).

Teacher(s)

Mgr. Karel Souček, Ph.D. (lecturer)
prof. RNDr. Eva Bártová, Ph.D., DSc. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
Mgr. Pavla Gajdušková, Ph.D. (lecturer)
Mgr. Eva Slabáková, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)

Guaranteed by

prof. RNDr. Alois Kozubík, CSc.
Department of Experimental Biology – Biology Section – Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.

Timetable

Tue 17:00–18:50 BFU

Prerequisites

No limitation for enrollment.

Course Enrolment Limitations

The course is also offered to the students of the fields other than those the course is directly associated with.

fields of study / plans the course is directly associated with

• General Biology (programme PřF, M-BI, specialization Physiology of Animals)

Course objectives

At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.

Syllabus

• * Flow cytometry general introduction - basic principles and history (Karel Souček)
• * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
• * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
• Flow cytometric methods in cell death analysis (Alena Vaculova)
• * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
• * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
• * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
• * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
• * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)

Literature

• Literatura: Teorie- Flow Cytometry: Instrumentation and Data Analysis (M.A. Van Dilla, P.N. Dean, O.D. Laerum, M.R. Melamed, eds.), Academic Press, London, 1985 Practical Flow Cytometry 3nd edition (1994),H. Shapiro: Alan R. Liss, New York Metody- The

Teaching methods

The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.

Assessment methods

Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.

Language of instruction

Czech

Further comments (probably available only in Czech)

Study Materials
The course is taught annually.

Listed among pre-requisites of other courses

• Bi9393en Analytical Cytometry
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• Enrolment Statistics (Autumn 2010, recent)
  
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