Bi9393 Analytical cytometry

Faculty of Science
autumn 2021
Extent and Intensity
2/0/0. 2 credit(s) (fasci plus compl plus > 4). Type of Completion: zk (examination).
Taught online.
Mgr. Karel Souček, Ph.D. (lecturer)
Mgr. Soňa Legartová, Ph.D. (lecturer)
doc. Mgr. Lukáš Kubala, Ph.D. (lecturer)
doc. RNDr. Alena Hyršlová Vaculová, Ph.D. (lecturer)
prof. Mgr. Vítězslav Bryja, Ph.D. (lecturer)
Guaranteed by
prof. Mgr. Vítězslav Bryja, Ph.D.
Department of Experimental Biology - Biology Section - Faculty of Science
Contact Person: Mgr. Karel Souček, Ph.D.
Supplier department: Department of Experimental Biology - Biology Section - Faculty of Science
Tue 14:00–15:50 BFU
No limitation for enrollment.
Course Enrolment Limitations
The course is also offered to the students of the fields other than those the course is directly associated with.
fields of study / plans the course is directly associated with
there are 6 fields of study the course is directly associated with, display
Course objectives
At the end of this course students should be able to: - understand principles and applications of modern methodologies of analytical cytometry such is flow cytometry, sorting, digital microscopy and microarrays; - understand basic principles of data analysis, their interpretation and presentation.
Learning outcomes
The student will learn detail principles of flow cytometry and fluorescent microscopy including practical applications of these methodologies.
  • * Flow cytometry general introduction - basic principles and history (Karel Souček)
  • * Principles of flow cytometry and sorting (Karel Souček) -fluorescence -excitation sources, optical systems and detection of fluorescence -signal processing and compensation -data visualization, analysis and presentation
  • * Biological application of flow cytometry (Karel Souček) -cell biology (nucleic acid analysis, cell function analysis, principles and application of multiparametric methods) -cytogenetics -hydrobiology
  • Flow cytometric methods in cell death analysis (Alena Vaculova)
  • * Application of flow cytometry in clinical immunology and hematology (Lukáš Kubala)
  • * Principles of digital microscopy (Eva Bártová) -fluorescent microscopy, confocal microscopy, temporally resolved -digital microscopy, time/frequency resolved digital microscopy
  • * Clinical and biological applications of digital microscopy (Eva Bártová) -chromosomal and gene analysis -metabolism and cellular compartments analysis (pH, ions) -FRET and FRAP applications
  • * Microarray analysis principles (Pavla Gajdušková) -DNA, RNA arrays -expression, gene and mutational arrays
  • * Biological and clinical application of microarrays and data analysis (Pavla Gajdušková) -practical examples of application in diagnostic and basic research * miRNA arrays (Eva Lincová)
    recommended literature
  • Practical Flow Cytometry
  • Flow Cytometry: Instrumentation and Data Analysis
  • - The Handbook of Flow Cytometry Methods - Current Protocols in Cytometry
Teaching methods
The lectures and discussions of the specialist with students are two most important parts of the course. The students will learn the principles of individual methodologies, and basic principles of data analysis. Practical demonstration of the instruments and equipments for flow cytometry and digital microscopy at the Institute of Biophysics in Brno is also included within the course.
Assessment methods
Course consist of lectures and two practical seminars. Every student will present short seminar. Form of exam is written test.
Language of instruction
Further comments (probably available only in Czech)
Study Materials
The course is taught annually.
Information on course enrolment limitations: Na předmět se vztahuje povinnost registrace, bez registrace může být znemožněn zápis předmětu!
The course is also listed under the following terms Autumn 2007 - for the purpose of the accreditation, Autumn 2010 - only for the accreditation, Autumn 2006, Autumn 2007, Autumn 2008, Autumn 2009, Autumn 2010, Autumn 2011, Autumn 2011 - acreditation, Autumn 2012, Autumn 2013, Autumn 2014, Autumn 2015, Autumn 2016, autumn 2017, Autumn 2018, Autumn 2019, Autumn 2020.
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